Summary: This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene encodes an enzyme that may be mainly involved in the synthesis of the first N-acetyllactosamine unit of poly-N-acetyllactosamine chains. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010].
shRNA Design:
These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service.
Performance Guranteed:
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
* Delivery time in business days.Occasional delay may occur due to complexity of the constructs.
shRNA Citations:
Identification and Cytoprotective Function of a Novel Nestin Isoform, Nes-S, in Dorsal Root Ganglia Neurons, Peng-Han Su, Chih-Cheng Chen, Ya-Fan Chang, Zong-Ruei Wong, Kai-Wei Chang, Bu-Miin Huang, and Hsi-Yuan Yang,
J. Biol. Chem., Mar 2013; 288: 8391 - 8404.
[NES] Runx1 and p21 synergistically limit the extent of hair follicle stem cell quiescence in vivo, Jayhun Lee, Charlene S. L. Hoi, Karin C. Lilja, Brian S. White, Song Eun Lee, David Shalloway, and Tudorita Tumbar,
PNAS, Mar 2013; 110: 4634 - 4639.
[CDKN2B ] Role of TRPM2 in cell proliferation and susceptibility to oxidative stress, Shu-jen Chen, Wenyi Zhang, Qin Tong, Kathleen Conrad, Iwona Hirschler-Laszkiewicz, Michael Bayerl, Jason K. Kim, Joseph Y. Cheung, and Barbara A. Miller,
Am J Physiol Cell Physiol, Mar 2013; 304: C548 - C560.
[FOXO3a ] Tubulin Acetyltransferase aTAT1 Destabilizes Microtubules Independently of Its Acetylation Activity, Nereo Kalebic, Concepcion Martinez, Emerald Perlas, Philip Hublitz, Daniel Bilbao-Cortes, Karol Fiedorczuk, Annapaola Andolfo, and Paul A. Heppenstall,
Mol. Cell. Biol., Mar 2013; 33: 1114 - 1123.
[ATAT1] YM-155 Potentiates the Effect of ABT-737 in Malignant Human Glioma Cells via Survivin and Mcl-1 Downregulation in an EGFR-Dependent Context, Esther P. Jane, Daniel R. Premkumar, Joseph D. DiDomenico, Bo Hu, Shi-Yuan Cheng, and Ian F. Pollack,
Mol. Cancer Ther., Mar 2013; 12: 326 - 338.
[MCL1] Fsp27/CIDEC is a CREB target gene induced during early fasting in liver and regulated by FA oxidation rate, Anna Vilà-Brau, Ana Luísa De Sousa-Coelho, Joana F. Gonçalves, Diego Haro, and Pedro F. Marrero,
J. Lipid Res., Mar 2013; 54: 592 - 601.
[HMGCS2 ] Inhibition of prolyl 4-hydroxylase, beta polypeptide (P4HB) attenuates temozolomide resistance in malignant glioma via the endoplasmic reticulum stress response (ERSR) pathways, Stella Sun, Derek Lee, Amy S.W. Ho, Jenny K.S. Pu, X.Q. Zhang, Nikki P. Lee, Philip J.R. Day, W.M. Lui, C.F. Fung, and Gilberto K.K. Leung,
Neuro Oncology, Feb 2013; 10.1093/neuonc/not005.
[P4HB] Identification of RNF8 as a Ubiquitin Ligase Involved in Targeting the p12 Subunit of DNA Polymerase for Degradation in Response to DNA Damage, Sufang Zhang, Yajing Zhou, Ali Sarkeshik, John R. Yates, III, Timothy M. Thomson, Zhongtao Zhang, Ernest Y. C. Lee, and Marietta Y. W. T. Lee,
J. Biol. Chem., Feb 2013; 288: 2941 - 2950.
[RNF8] PBX3 is an important cofactor of HOXA9 in leukemogenesis, Zejuan Li, Zhiyu Zhang, Yuanyuan Li, Stephen Arnovitz, Ping Chen, Hao Huang, Xi Jiang, Gia-Ming Hong, Rejani B. Kunjamma, Haomin Ren, Chunjiang He, Chong-Zhi Wang, Abdel G. Elkahloun, Peter J. M. Valk, Konstanze Döhner, Mary Beth Neilly, Lars Bullinger, Ruud Delwel, Bob Löwenberg, Paul P. Liu, Richard Morgan, Janet D. Rowley, Chun-Su Yuan, and Jianjun Chen,
Blood, Feb 2013; 121: 1422 - 1431.
[Pbx3] Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development, Kee K. Kim, Joseph Nam, Yoh-suke Mukouyama, and Sachiyo Kawamoto,
J. Cell Biol., Feb 2013; 200: 443 - 458.
[Rbfox3]
* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping
