Summary: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011].
These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service.
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
* Delivery time in business days.Occasional delay may occur due to complexity of the constructs.
All shRNA Citations:
Silencing SATB1 influences cell invasion, migration, proliferation, and drug resistance in nasopharyngeal carcinoma, Ye, CS;Zhou, DN;Yang, QQ;Deng, YF;,
Int J Clin Exp Pathol Apr 2014
[SATB1] ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism, Yang, Y;Ahn, YH;Chen, Y;Tan, X;Guo, L;Gibbons, DL;Ungewiss, C;Peng, DH;Liu, X;Lin, SH;Thilaganathan, N;Wistuba, II;Rodriguez-Canales, J;McLendon, G;Creighton, CJ;Kurie, JM;,
J. Clin. Invest. April 2014
[FOG2] HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation, Hu, B;Li, S;Zhang, X;Zheng, X;,
Nucleic Acids Res. April 2014
[USP7] The transcription factor GLI1 interacts with SMAD proteins to modulate TGFß-induced gene expression in a PCAF-dependent manner, Nye, MD;Almada, LL;Fernandez-Barrena, MG;Marks, DL;Elsawa, SF;Vrabel, A;Tolosa, EJ;Ellenrieder, V;Fernandez-Zapico, ME;,
J. Biol. Chem. April 2014
[SMAD4] The transcription factor GLI1 interacts with SMAD proteins to modulate TGFß-induced gene expression in a PCAF-dependent manner, Nye, MD;Almada, LL;Fernandez-Barrena, MG;Marks, DL;Elsawa, SF;Vrabel, A;Tolosa, EJ;Ellenrieder, V;Fernandez-Zapico, ME;,
J. Biol. Chem. April 2014
[SMAD2] Culture dimensionality influences the resistance of glioblastoma stem-like cells to multikinase inhibitors, Fernandez-Fuente, G;Mollinedo, P;Grande, L;Vazquez-Barquero, A;Fernandez-Luna, JL;,
Mol. Cancer Ther. April 2014
[PDGFRA] HSV-2 Increases TLR4-Dependent Phosphorylated IRFs and IFN-ß Induction in Cervical Epithelial Cells, Liu, H;Chen, K;Feng, W;Guo, J;Li, H;,
PLoS ONE e94806 9 4. April 2014
[TLR4] Chymase Mediates Injury and Mitochondrial Damage in Cardiomyocytes during Acute Ischemia/Reperfusion in the Dog, Zheng, J;Wei, CC;Hase, N;Shi, K;Killingsworth, CR;Litovsky, SH;Powell, PC;Kobayashi, T;Ferrario, CM;Rab, A;Aban, I;Collawn, JF;Dell'italia, LJ;,
PLoS ONE e94732 9 4. April 2014
[NR4A1] Primary cilium regulates CaV1.2 expression through Wnt signaling, Muntean, BS;Jin, X;Williams, FE;Nauli, SM;,
J. Cell. Physiol. April 2014
[CACNA1C] A DERL3-associated defect in the degradation of SLC2A1 mediates the Warburg effect, Lopez-Serra, P;Marcilla, M;Villanueva, A;Ramos-Fernandez, A;Palau, A;Leal, L;Wahi, JE;Setien-Baranda, F;Szczesna, K;Moutinho, C;Martinez-Cardus, A;Heyn, H;Sandoval, J;Puertas, S;Vidal, A;Sanjuan, X;Martinez-Balibrea, E;Viñals, F;Perales, JC;Bramsem, JB;Ørntoft, TF;Andersen, CL;Tabernero, J;McDermott, U;Boxer, MB;Heiden, MG;Albar, JP;Esteller, M;,
Nat Commun 3608 5 . April 2014
* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping