Summary: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. [provided by RefSeq, Aug 2011].
These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service.
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
* Delivery time in business days.Occasional delay may occur due to complexity of the constructs.
Identification and Cytoprotective Function of a Novel Nestin Isoform, Nes-S, in Dorsal Root Ganglia Neurons, Peng-Han Su, Chih-Cheng Chen, Ya-Fan Chang, Zong-Ruei Wong, Kai-Wei Chang, Bu-Miin Huang, and Hsi-Yuan Yang,
J. Biol. Chem., Mar 2013; 288: 8391 - 8404.
[NES] Runx1 and p21 synergistically limit the extent of hair follicle stem cell quiescence in vivo, Jayhun Lee, Charlene S. L. Hoi, Karin C. Lilja, Brian S. White, Song Eun Lee, David Shalloway, and Tudorita Tumbar,
PNAS, Mar 2013; 110: 4634 - 4639.
[CDKN2B ] Role of TRPM2 in cell proliferation and susceptibility to oxidative stress, Shu-jen Chen, Wenyi Zhang, Qin Tong, Kathleen Conrad, Iwona Hirschler-Laszkiewicz, Michael Bayerl, Jason K. Kim, Joseph Y. Cheung, and Barbara A. Miller,
Am J Physiol Cell Physiol, Mar 2013; 304: C548 - C560.
[FOXO3a ] Tubulin Acetyltransferase aTAT1 Destabilizes Microtubules Independently of Its Acetylation Activity, Nereo Kalebic, Concepcion Martinez, Emerald Perlas, Philip Hublitz, Daniel Bilbao-Cortes, Karol Fiedorczuk, Annapaola Andolfo, and Paul A. Heppenstall,
Mol. Cell. Biol., Mar 2013; 33: 1114 - 1123.
[ATAT1] YM-155 Potentiates the Effect of ABT-737 in Malignant Human Glioma Cells via Survivin and Mcl-1 Downregulation in an EGFR-Dependent Context, Esther P. Jane, Daniel R. Premkumar, Joseph D. DiDomenico, Bo Hu, Shi-Yuan Cheng, and Ian F. Pollack,
Mol. Cancer Ther., Mar 2013; 12: 326 - 338.
[MCL1] Fsp27/CIDEC is a CREB target gene induced during early fasting in liver and regulated by FA oxidation rate, Anna Vilà-Brau, Ana Luísa De Sousa-Coelho, Joana F. Gonçalves, Diego Haro, and Pedro F. Marrero,
J. Lipid Res., Mar 2013; 54: 592 - 601.
[HMGCS2 ] Inhibition of prolyl 4-hydroxylase, beta polypeptide (P4HB) attenuates temozolomide resistance in malignant glioma via the endoplasmic reticulum stress response (ERSR) pathways, Stella Sun, Derek Lee, Amy S.W. Ho, Jenny K.S. Pu, X.Q. Zhang, Nikki P. Lee, Philip J.R. Day, W.M. Lui, C.F. Fung, and Gilberto K.K. Leung,
Neuro Oncology, Feb 2013; 10.1093/neuonc/not005.
[P4HB] Identification of RNF8 as a Ubiquitin Ligase Involved in Targeting the p12 Subunit of DNA Polymerase for Degradation in Response to DNA Damage, Sufang Zhang, Yajing Zhou, Ali Sarkeshik, John R. Yates, III, Timothy M. Thomson, Zhongtao Zhang, Ernest Y. C. Lee, and Marietta Y. W. T. Lee,
J. Biol. Chem., Feb 2013; 288: 2941 - 2950.
[RNF8] PBX3 is an important cofactor of HOXA9 in leukemogenesis, Zejuan Li, Zhiyu Zhang, Yuanyuan Li, Stephen Arnovitz, Ping Chen, Hao Huang, Xi Jiang, Gia-Ming Hong, Rejani B. Kunjamma, Haomin Ren, Chunjiang He, Chong-Zhi Wang, Abdel G. Elkahloun, Peter J. M. Valk, Konstanze Döhner, Mary Beth Neilly, Lars Bullinger, Ruud Delwel, Bob Löwenberg, Paul P. Liu, Richard Morgan, Janet D. Rowley, Chun-Su Yuan, and Jianjun Chen,
Blood, Feb 2013; 121: 1422 - 1431.
[Pbx3] Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development, Kee K. Kim, Joseph Nam, Yoh-suke Mukouyama, and Sachiyo Kawamoto,
J. Cell Biol., Feb 2013; 200: 443 - 458.
* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping