Summary: The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. [provided by RefSeq, Jul 2008].
shRNA Design:
These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service.
Performance Guranteed:
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
* Delivery time in business days.Occasional delay may occur due to complexity of the constructs.
shRNA Citations:
Identification and Cytoprotective Function of a Novel Nestin Isoform, Nes-S, in Dorsal Root Ganglia Neurons, Peng-Han Su, Chih-Cheng Chen, Ya-Fan Chang, Zong-Ruei Wong, Kai-Wei Chang, Bu-Miin Huang, and Hsi-Yuan Yang,
J. Biol. Chem., Mar 2013; 288: 8391 - 8404.
[NES] Runx1 and p21 synergistically limit the extent of hair follicle stem cell quiescence in vivo, Jayhun Lee, Charlene S. L. Hoi, Karin C. Lilja, Brian S. White, Song Eun Lee, David Shalloway, and Tudorita Tumbar,
PNAS, Mar 2013; 110: 4634 - 4639.
[CDKN2B ] Role of TRPM2 in cell proliferation and susceptibility to oxidative stress, Shu-jen Chen, Wenyi Zhang, Qin Tong, Kathleen Conrad, Iwona Hirschler-Laszkiewicz, Michael Bayerl, Jason K. Kim, Joseph Y. Cheung, and Barbara A. Miller,
Am J Physiol Cell Physiol, Mar 2013; 304: C548 - C560.
[FOXO3a ] Tubulin Acetyltransferase aTAT1 Destabilizes Microtubules Independently of Its Acetylation Activity, Nereo Kalebic, Concepcion Martinez, Emerald Perlas, Philip Hublitz, Daniel Bilbao-Cortes, Karol Fiedorczuk, Annapaola Andolfo, and Paul A. Heppenstall,
Mol. Cell. Biol., Mar 2013; 33: 1114 - 1123.
[ATAT1] YM-155 Potentiates the Effect of ABT-737 in Malignant Human Glioma Cells via Survivin and Mcl-1 Downregulation in an EGFR-Dependent Context, Esther P. Jane, Daniel R. Premkumar, Joseph D. DiDomenico, Bo Hu, Shi-Yuan Cheng, and Ian F. Pollack,
Mol. Cancer Ther., Mar 2013; 12: 326 - 338.
[MCL1] Fsp27/CIDEC is a CREB target gene induced during early fasting in liver and regulated by FA oxidation rate, Anna Vilà-Brau, Ana Luísa De Sousa-Coelho, Joana F. Gonçalves, Diego Haro, and Pedro F. Marrero,
J. Lipid Res., Mar 2013; 54: 592 - 601.
[HMGCS2 ] Inhibition of prolyl 4-hydroxylase, beta polypeptide (P4HB) attenuates temozolomide resistance in malignant glioma via the endoplasmic reticulum stress response (ERSR) pathways, Stella Sun, Derek Lee, Amy S.W. Ho, Jenny K.S. Pu, X.Q. Zhang, Nikki P. Lee, Philip J.R. Day, W.M. Lui, C.F. Fung, and Gilberto K.K. Leung,
Neuro Oncology, Feb 2013; 10.1093/neuonc/not005.
[P4HB] Identification of RNF8 as a Ubiquitin Ligase Involved in Targeting the p12 Subunit of DNA Polymerase for Degradation in Response to DNA Damage, Sufang Zhang, Yajing Zhou, Ali Sarkeshik, John R. Yates, III, Timothy M. Thomson, Zhongtao Zhang, Ernest Y. C. Lee, and Marietta Y. W. T. Lee,
J. Biol. Chem., Feb 2013; 288: 2941 - 2950.
[RNF8] PBX3 is an important cofactor of HOXA9 in leukemogenesis, Zejuan Li, Zhiyu Zhang, Yuanyuan Li, Stephen Arnovitz, Ping Chen, Hao Huang, Xi Jiang, Gia-Ming Hong, Rejani B. Kunjamma, Haomin Ren, Chunjiang He, Chong-Zhi Wang, Abdel G. Elkahloun, Peter J. M. Valk, Konstanze Döhner, Mary Beth Neilly, Lars Bullinger, Ruud Delwel, Bob Löwenberg, Paul P. Liu, Richard Morgan, Janet D. Rowley, Chun-Su Yuan, and Jianjun Chen,
Blood, Feb 2013; 121: 1422 - 1431.
[Pbx3] Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development, Kee K. Kim, Joseph Nam, Yoh-suke Mukouyama, and Sachiyo Kawamoto,
J. Cell Biol., Feb 2013; 200: 443 - 458.
[Rbfox3]
* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping
