Summary: PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul
These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service.
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at email@example.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
* Delivery time in business days.Occasional delay may occur due to complexity of the constructs.
All shRNA Citations:
Silencing SATB1 influences cell invasion, migration, proliferation, and drug resistance in nasopharyngeal carcinoma, Ye, CS;Zhou, DN;Yang, QQ;Deng, YF;,
Int J Clin Exp Pathol Apr 2014
[SATB1] ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism, Yang, Y;Ahn, YH;Chen, Y;Tan, X;Guo, L;Gibbons, DL;Ungewiss, C;Peng, DH;Liu, X;Lin, SH;Thilaganathan, N;Wistuba, II;Rodriguez-Canales, J;McLendon, G;Creighton, CJ;Kurie, JM;,
J. Clin. Invest. April 2014
[FOG2] HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation, Hu, B;Li, S;Zhang, X;Zheng, X;,
Nucleic Acids Res. April 2014
[USP7] The transcription factor GLI1 interacts with SMAD proteins to modulate TGFß-induced gene expression in a PCAF-dependent manner, Nye, MD;Almada, LL;Fernandez-Barrena, MG;Marks, DL;Elsawa, SF;Vrabel, A;Tolosa, EJ;Ellenrieder, V;Fernandez-Zapico, ME;,
J. Biol. Chem. April 2014
[SMAD4] The transcription factor GLI1 interacts with SMAD proteins to modulate TGFß-induced gene expression in a PCAF-dependent manner, Nye, MD;Almada, LL;Fernandez-Barrena, MG;Marks, DL;Elsawa, SF;Vrabel, A;Tolosa, EJ;Ellenrieder, V;Fernandez-Zapico, ME;,
J. Biol. Chem. April 2014
[SMAD2] Culture dimensionality influences the resistance of glioblastoma stem-like cells to multikinase inhibitors, Fernandez-Fuente, G;Mollinedo, P;Grande, L;Vazquez-Barquero, A;Fernandez-Luna, JL;,
Mol. Cancer Ther. April 2014
[PDGFRA] HSV-2 Increases TLR4-Dependent Phosphorylated IRFs and IFN-ß Induction in Cervical Epithelial Cells, Liu, H;Chen, K;Feng, W;Guo, J;Li, H;,
PLoS ONE e94806 9 4. April 2014
[TLR4] Chymase Mediates Injury and Mitochondrial Damage in Cardiomyocytes during Acute Ischemia/Reperfusion in the Dog, Zheng, J;Wei, CC;Hase, N;Shi, K;Killingsworth, CR;Litovsky, SH;Powell, PC;Kobayashi, T;Ferrario, CM;Rab, A;Aban, I;Collawn, JF;Dell'italia, LJ;,
PLoS ONE e94732 9 4. April 2014
[NR4A1] Primary cilium regulates CaV1.2 expression through Wnt signaling, Muntean, BS;Jin, X;Williams, FE;Nauli, SM;,
J. Cell. Physiol. April 2014
[CACNA1C] A DERL3-associated defect in the degradation of SLC2A1 mediates the Warburg effect, Lopez-Serra, P;Marcilla, M;Villanueva, A;Ramos-Fernandez, A;Palau, A;Leal, L;Wahi, JE;Setien-Baranda, F;Szczesna, K;Moutinho, C;Martinez-Cardus, A;Heyn, H;Sandoval, J;Puertas, S;Vidal, A;Sanjuan, X;Martinez-Balibrea, E;Viñals, F;Perales, JC;Bramsem, JB;Ørntoft, TF;Andersen, CL;Tabernero, J;McDermott, U;Boxer, MB;Heiden, MG;Albar, JP;Esteller, M;,
Nat Commun 3608 5 . April 2014
* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping