Custom Stable Cell Line Development

Taking advantage of our largest commercial collection of human & mouse cDNA clones, 12,000 over-expression lysates and 5,000 purified full length human proteins in HEK293 cells, OriGene now provides custom stable cell line development service with speed and quality in various applications

• Stable cell line for recombinant protein production: monoclonal antibodies, growth factor and other soluble proteins. We will clone your target gene into our bicistronic DNA amplification vector to increase gene copy number up to several hundred in the final clone
• Stable cell line for cell-based assay: GPCRs, ion channel and kinases (RTKs). We will use our proprietary bicistronic vector, inducible vector or your preferred vector to achieve the best receptor expression on cell surface.

Our service includes:

• Complete DNA sequence verification
• Colony isolation, clone screening using ELISA, Western, or cell based assay of your interest, such as intracellular calcium, IP1, intracellular cAMP, thallium flux or membrane potential assay, et al..
• The best clone will be banked as Master Cell Bank and fully validated as your request.

Please use to submit your request. OriGene will contact you within 48 hours to provide feasibility and cost assessment.

Contact OriGene Technical Support if you have any questions about our service or how to fill out the form.
Phone: 888-267-4436 (business hours: 9AM-7PM ET)
E-mail: techsupport@origene.com

Related Products & Services:

• TrueORF cDNA clones
• VERIFY Tagged Antigen™ (over-expression cell lysates)
• Purified Human Proteins