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Home Recombinant Human Proteins All DNMT1 Proteins

DNMT1 (NM_001130823) Purified Human Protein

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Cat. No. Description Datasheet Price Availability*  
TP326414 Recombinant protein of human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1, 20 µg (Larger pack size?) 680 In Stock
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Also for DNMT1 (NM_001130823)
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OriGene Data
Species:Human Expression Host:HEK293 cells
Expression cDNA Clone or AA Sequence
Recombinant protein was produced with TrueORF clone, RC226414. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag:C-terminal MYC/DDK Predicted MW:184.6 kDa
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>50 ug/mL as determined by microplate BCA method
Buffer:25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
BioActivity: DNMT1 activity verified in a biochemical assay: DNMT1 (DNA (cytosine-5-)-methyltransferase 1) (TP326414) is a key methyltransferase that is responsible for maintaining methylation patterns established in development. DNMT1 preferentially methylates hemi-methylated CpG di-nucleotides and associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand. Varying concentrations of DNMT1 were added to a microplate containing a bound methyltransferase substrate. After incubation, the resulting methylated DNA residues were detected immunologically and a colorimetric signal was generated and measured.
Protein Families: Transcription FactorsDruggable Genome
Protein Pathways: Cysteine and methionine metabolismMetabolic pathways
Protein Data
Protein Image
BioActivity Data
BioActivity Image
 

Reference Data
RefSeq: NP_001124295 RefSeq Size: RefSeq ORF: 4899
LocusID: 1786 Cytogenetic: 19p13.2  
Synonyms : ADCADN; AIM; CXXC9; DNMT; HSN1E; MCMT
Summary: DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008].
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

 

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