PAK1 (NM_001128620) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC225947. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 61.5 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein KinaseStem cell - PluripotencyDruggable Genome Protein Pathways: MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayAxon guidanceFocal adhesionNatural killer cell mediated cytotoxicityMore Pathways >>

Protein Data

RefSeq: NP_001122092	RefSeq Size:	RefSeq ORF: 1662
LocusID: 5058	Cytogenetic: 11q13
Synonyms : PAKalpha
Summary: This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 1 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 1 Validated Antibodies • PAK1 Rabbit Polyclonal (Internal) Antibody • PAK1 Rabbit Polyclonal (Internal) Antibody • PAK1/2/3 Rabbit Polyclonal (C-Terminus) Antibody • Purified Goat Polyclonal Antibody against PAK1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • PAK1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • PAK1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Pak1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • PAK1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Pak1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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