RUNX1 (NM_001001890) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC223854. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 48.6 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. BioActivity: RUNX1 Activity verified in a DNA-binding assay: Protein Families: ES Cell Differentiation/IPSTranscription FactorsDruggable Genome Protein Pathways: Pathways in cancerChronic myeloid leukemiaAcute myeloid leukemia

Protein Data BioActivity Data

RefSeq: NP_001001890	RefSeq Size: 7288	RefSeq ORF: 1362
LocusID: 861	Cytogenetic: 21q22.3
Synonyms : AML1; AML1-EVI-1; AMLCR1; CBFA2; EVI-1; PEBP2aB
Summary: Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens runt-related transcription factor 1 (RUNX1), transcript variant 2 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens runt-related transcription factor 1 (RUNX1), transcript variant 2 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of runt-related transcription factor 1 (RUNX1), transcript variant 2 Validated Antibodies • Rabbit Polyclonal RUNX1 Antibody • Rabbit polyclonal AML1 / RUNX1 (Ab-276) antibody • Rabbit polyclonal AML1 / RUNX1 (Ab-303) antibody • Rabbit polyclonal AML1 / RUNX1 (Ab-435) antibody • Rabbit polyclonal AML1 / RUNX1 (Ser435) antibody(Phospho-specific) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • RUNX1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • RUNX1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • RUNX1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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