DOCK8 (NM_203447) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC222789. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 238.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways:

Protein Data

RefSeq: NP_982272	RefSeq Size: 7257	RefSeq ORF: 6096
LocusID: 81704	Cytogenetic: 9p24.3
Synonyms : MRD2; ZIR8
Summary: This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jun 2010].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens dedicator of cytokinesis 8 (DOCK8), transcript variant 1 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens dedicator of cytokinesis 8 (DOCK8), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens dedicator of cytokinesis 8 (DOCK8), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens dedicator of cytokinesis 8 (DOCK8), transcript variant 1 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of dedicator of cytokinesis 8 (DOCK8) Validated Antibodies • Purified DOCK8 mouse monoclonal antibody,clone 2C11 • Purified DOCK8 mouse monoclonal antibody,clone 5C4 • Purified DOCK8 mouse monoclonal antibody,clone 4F3 • Carrier-free (BSA/glycerol-free) DOCK8 mouse monoclonal antibody,clone 2C11 • Carrier-free (BSA/glycerol-free) DOCK8 mouse monoclonal antibody,clone 5C4 • Carrier-free (BSA/glycerol-free) DOCK8 mouse monoclonal antibody,clone 4F3 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • DOCK8 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • DOCK8 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Dock8 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • DOCK8 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Dock8 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene DOCK8

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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