HAO1 (NM_017545) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC216834. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 40.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways: Glyoxylate and dicarboxylate metabolismMetabolic pathways

Protein Data

RefSeq: NP_060015	RefSeq Size: 1746	RefSeq ORF: 1113
LocusID: 54363	Cytogenetic: 20p12
Synonyms : GOX; GOX1; HAOX1
Summary: This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens hydroxyacid oxidase (glycolate oxidase) 1 (HAO1) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens hydroxyacid oxidase (glycolate oxidase) 1 (HAO1) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens hydroxyacid oxidase (glycolate oxidase) 1 (HAO1) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of hydroxyacid oxidase (glycolate oxidase) 1 (HAO1) Validated Antibodies • Purified HAO1 mouse monoclonal antibody, clone 5C3 • Purified HAO1 mouse monoclonal antibody, clone 3E7 • Purified HAO1 mouse monoclonal antibody, clone 5F4 • Purified HAO1 mouse monoclonal antibody, clone 5A1 • Purified HAO1 mouse monoclonal antibody, clone 6B3 • Carrier-free (BSA/glycerol-free) HAO1 mouse monoclonal antibody, clone 5C3 • Carrier-free (BSA/glycerol-free) HAO1 mouse monoclonal antibody, clone 3E7 • Carrier-free (BSA/glycerol-free) HAO1 mouse monoclonal antibody, clone 5F4 • Carrier-free (BSA/glycerol-free) HAO1 mouse monoclonal antibody, clone 5A1 • Carrier-free (BSA/glycerol-free) HAO1 mouse monoclonal antibody, clone 6B3 • Purified HAO1 mouse monoclonal antibody,clone UMAB117 • Purified HAO1 mouse monoclonal antibody,clone UMAB117 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • HAO1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • HAO1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Hao1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • HAO1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Hao1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene HAO1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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