PLA2G1B (NM_000928) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC216089. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 16.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Secreted ProteinDruggable Genome Protein Pathways: Glycerophospholipid metabolismEther lipid metabolismArachidonic acid metabolismLinoleic acid metabolismalpha-Linolenic acid metabolismMetabolic pathwaysMore Pathways >>

Protein Data

RefSeq: NP_000919	RefSeq Size: 585	RefSeq ORF: 447
LocusID: 5319	Cytogenetic: 12q23
Synonyms : PLA2; PLA2A; PPLA2
Summary: Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]) .[supplied by OMIM, Mar 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens phospholipase A2, group IB (pancreas) (PLA2G1B) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens phospholipase A2, group IB (pancreas) (PLA2G1B) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens phospholipase A2, group IB (pancreas) (PLA2G1B) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of phospholipase A2, group IB (pancreas) (PLA2G1B) Validated Antibodies • Purified Goat Polyclonal Antibody against PLA2G1B • Rabbit monoclonal antibody against PLA2G1B(clone EPR5186) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • PLA2G1B - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • PLA2G1B - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Pla2g1b - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • PLA2G1B - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Pla2g1b - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene PLA2G1B

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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