GH1 (NM_000515) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC215644. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 24.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Secreted ProteinDruggable Genome Protein Pathways: Cytokine-cytokine receptor interactionNeuroactive ligand-receptor interactionJak-STAT signaling pathway

Protein Data

RefSeq: NP_000506	RefSeq Size: 822	RefSeq ORF: 654
LocusID: 2688	Cytogenetic: 17q24.2
Synonyms : GH; GH-N; GHN; hGH-N; IGHD1B
Summary: The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq, Jul

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens growth hormone 1 (GH1), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human growth hormone 1 (GH1), transcript variant 1, mGFP tagged • GFP-tagged ORF clone of Homo sapiens growth hormone 1 (GH1), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human growth hormone 1 (GH1), transcript variant 1 , Myc-DDK-tagged • Myc-DDK-tagged ORF clone of Homo sapiens growth hormone 1 (GH1), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of growth hormone 1 (GH1), transcript variant 1 Validated Antibodies • Rabbit polyclonal anti-GH1 antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • GH1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • GH1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • GH1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene GH1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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