DAPK1 (NM_004938) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC215423. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 159.9 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein KinaseDruggable Genome Protein Pathways: Pathways in cancerBladder cancer

Protein Data

RefSeq: NP_004929	RefSeq Size: 5910	RefSeq ORF: 4296
LocusID: 1612	Cytogenetic: 9q21.33
Synonyms : DAPK
Summary: Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens death-associated protein kinase 1 (DAPK1) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens death-associated protein kinase 1 (DAPK1) as transfection-ready DNA • Lenti ORF clone of Human death-associated protein kinase 1 (DAPK1) , Myc-DDK-tagged • Lenti ORF clone of Human death-associated protein kinase 1 (DAPK1), mGFP tagged Related Lysate from OriGene • Transient overexpression lysate of death-associated protein kinase 1 (DAPK1) Validated Antibodies • Rabbit polyclonal anti-DAPK1 antibody • Rabbit Monoclonal antibody against DAPK1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • DAPK1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • DAPK1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Dapk1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • DAPK1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Dapk1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene DAPK1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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