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Home Recombinant Human Proteins All EFEMP1 Proteins

EFEMP1 (NM_004105) Purified Human Protein

Specifications Citations (1) Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP314953 Recombinant protein of human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1, 20 ug (Larger pack size?) 680 In Stock
TA503772 EFEMP1 mouse monoclonal antibody,clone 1D9 $379 In Stock
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Also for EFEMP1 (NM_004105)
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OriGene Data
Species:Human Expression Host:HEK293 cells
Expression cDNA Clone or AA Sequence
Recombinant protein was produced with TrueORF clone, RC214953. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag:C-terminal MYC/DDK Predicted MW:52.7 kDa
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology.
Concentration:>50 ug/mL as determined by microplate BCA method
Buffer:25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
BioActivity: : 200 ng of recombinant purified EFEMP1-myc were added to cultured glioma cells (CNS-1 rat glioma cell line) for 6h, and cells were further processed for quantitative RT-PCR.

Results showed increased expression of metalloproteases MMP2 and MMP9, in agreement with published results from Hu. et. al. (Mol. Cancer Res. (2009) 7:1756-1770)

Data kindly provided by Dr. Mariano S. Viapiano, Ohio State University.
Protein Families: Secreted Protein
Protein Data
Protein Image
BioActivity Data
BioActivity Image
 

Reference Data
RefSeq: NP_004096 RefSeq Size: 2742 RefSeq ORF: 1482
LocusID: 2202 Cytogenetic: 2p16  
Synonyms : DHRD; DRAD; FBLN3; FBNL; FLJ35535; MGC111353; MLVT; MTLV; S1-5;EGF-containing fibulin-like extracellular matrix protein 1; DRAD; FBLN3; FBNL; FLJ35535; MGC111353; MLVT; MTLV; S1-5; fibrillin-like; fibulin 3
Summary:
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

 

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