||Expression Host:||HEK293 cells
|Expression cDNA Clone or AA Sequence
Recombinant protein was produced with TrueORF clone, RC211013
. Click on the TrueORF clone link to view cDNA and protein sequences.
||Predicted MW:||84.3 kDa|
|Purity:||> 80% as determined by SDS-PAGE and Coomassie blue staining|
|Concentration:||>50 ug/mL as determined by microplate BCA method|
|Buffer:||25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.|
||Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
||BRAF kinase activity was measured in an HTRF® assay. Varying concentrations of BRAF were added to a reaction mix containing ATP and a biotinylated kinase substrate (HTRF substrate 2) and was incubated at 37C for phosphorylation. HTRF detection reagents were then added, the reaction was incubated for 30 minutes at room temperature. Time-resolved fluorescent signal (Delta R) was measured on a Flexstation 3 microplate reader.
||Protein KinaseDruggable Genome
||MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayVascular smooth muscle contractionFocal adhesionMore Pathways >>