DCK (NM_000788) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC210767. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 30.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Purine metabolismPyrimidine metabolism

Protein Data

RefSeq: NP_000779	RefSeq Size: 2460	RefSeq ORF: 783
LocusID: 1633	Cytogenetic: 4q13.3
Synonyms : MGC117410; MGC138632
Summary: Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens deoxycytidine kinase (DCK) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens deoxycytidine kinase (DCK) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens deoxycytidine kinase (DCK) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of deoxycytidine kinase (DCK) Validated Antibodies • Purified DCK mouse monoclonal antibody, clone 16E12 • Purified DCK mouse monoclonal antibody, clone 3F5 • Purified DCK mouse monoclonal antibody, clone 16G6 • Purified DCK mouse monoclonal antibody,clone 15E12 • Rabbit Polyclonal antibody to DCK (deoxycytidine kinase) • Carrier-free (BSA/glycerol-free) DCK mouse monoclonal antibody, clone 16E12 • Carrier-free (BSA/glycerol-free) DCK mouse monoclonal antibody, clone 3F5 • Carrier-free (BSA/glycerol-free) DCK mouse monoclonal antibody, clone 16G6 • Carrier-free (BSA/glycerol-free) DCK mouse monoclonal antibody,clone 15E12 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • DCK - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • DCK - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Dck - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • DCK - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Dck - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene DCK

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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