EFNA1 (NM_004428) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC210635. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 21.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Axon guidance

Protein Data

RefSeq: NP_004419	RefSeq Size: 1590	RefSeq ORF: 618
LocusID: 1942	Cytogenetic: 1q21
Synonyms : B61; ECKLG; EFL1; EPLG1; LERK-1; LERK1; TNFAIP4
Summary: This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens ephrin-A1 (EFNA1), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens ephrin-A1 (EFNA1), transcript variant 1 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of ephrin-A1 (EFNA1), transcript variant 1 Validated Antibodies • Rabbit Monoclonal antibody against Ephrin-A1 (EFNA1) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • EFNA1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • EFNA1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Efna1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • EFNA1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Efna1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene EFNA1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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