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OriGene protein in recent publications
Roles of Heparan Sulfate Sulfation in Dentinogenesis J. Biol. Chem., Apr 2012; 287: 12217 - 12229. [WNT10A ]
Antibody-based Protein Profiling of the Human Chromosome 21 Mol. Cell. Proteomics, Mar 2012; 11: M111.013458. [C21orf59 ]
Antibody-based Protein Profiling of the Human Chromosome 21 Mol. Cell. Proteomics, Mar 2012; 11: M111.013458. [C21orf56 ]
Anti-TIF1 antibodies (anti-p155) in adult patients with dermatomyositis: comparison of different diagnostic assays Ann Rheum Dis, Jan 2012; 10.1136/annrheumdis-2011-200871. [TRIM33 ]
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NPR1 (NM_000906) Purified Human Protein

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Cat. No. Ref. ID Description Price Availability*
TP309267 NM_000906 Purified recombinant protein of Homo sapiens natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A) (NPR1), expressed in human cells, 20 µg $680 Next day

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Also for NPR1 (NM_000906)
cDNA Clone shRNA/siRNA Mass Spec Std Lysate Antibody Service

OriGene Data
Expression Host:Human HEK293 cells Predicted MW:118.7 kDa
Protein Image Not Available
Expression cDNA Clone and Sequence HEK293 cells transient transfection with TrueORF clone, RC209267. Click on the TrueORF clone link to view cDNA clone sequence and protein sequence
Tag:C-terminal MYC/DDK** Anti-Myc/DDK antibody
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:> 50 ug/ml as determined by BCA
Preparation:HEK293T cells were transiently transfected with MegaTran Transfection Reagent (TT200002) and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Recombinant proteins were purified through anti-DDK affinity columns. Protein concentration was measured by BCA kit.
Buffer and Storage:10% glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
Protein Families: Protein KinaseDruggable Genome
Protein Pathways: Purine metabolismVascular smooth muscle contraction

Reference Data
RefSeq: NP_000897
 RefSeq Size: 4246 RefSeq ORF: 3186
Synonyms : ANPa; ANPRA; GUC2A; GUCY2A; NPRA
LocusID: 4881 Cytogenetic: 1q21  
Summary: Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).[supplied by OMIM].
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich