UBE2D2 (NM_181838) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC207283. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 13.5 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways: Ubiquitin mediated proteolysis

Protein Data

RefSeq: NP_862821	RefSeq Size: 2879	RefSeq ORF: 357
LocusID: 7322	Cytogenetic: 5q31.2
Synonyms : E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH5B
Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Jul 2008].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens ubiquitin-conjugating enzyme E2D 2 (UBE2D2), transcript variant 2 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens ubiquitin-conjugating enzyme E2D 2 (UBE2D2), transcript variant 2 as transfection-ready DNA • Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 2 (UBE2D2), transcript variant 2 , Myc-DDK-tagged • Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 2 (UBE2D2), transcript variant 2, mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens ubiquitin-conjugating enzyme E2D 2 (UBE2D2), transcript variant 2 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast) (UBE2D2), transcript variant 2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • UBE2D2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • UBE2D2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ube2d2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • UBE2D2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ube2d2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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