GRAP2 (NM_004810) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC206546. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 37.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: T cell receptor signaling pathway

Protein Data

RefSeq: NP_004801	RefSeq Size: 1552	RefSeq ORF: 993
LocusID: 9402	Cytogenetic: 22q13.2
Synonyms : GADS; GRAP-2; GRB2L; GRBLG; GrbX; Grf40; GRID; GRPL; Mona; P38
Summary: This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens GRB2-related adaptor protein 2 (GRAP2) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens GRB2-related adaptor protein 2 (GRAP2) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens GRB2-related adaptor protein 2 (GRAP2) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of GRB2-related adaptor protein 2 (GRAP2) Validated Antibodies • Purified Goat Polyclonal Antibody against GRAP2 / GRID / Grf40 • Rabbit polyclonal antibody to GRAP2 (GRB2-related adaptor protein 2) • Rabbit polyclonal anti-GRAP2 antibody • Purified GRAP2 mouse monoclonal antibody,clone 1G2 • Carrier-free (BSA/glycerol-free) GRAP2 mouse monoclonal antibody,clone 1G2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • GRAP2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • GRAP2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Grap2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • GRAP2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Grap2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene GRAP2

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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