DAK (NM_015533) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC201724. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 58.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Glycerolipid metabolismMetabolic pathwaysRIG-I-like receptor signaling pathway

Protein Data

RefSeq: NP_056348	RefSeq Size: 4248	RefSeq ORF: 1728
LocusID: 26007	Cytogenetic: 11q12.2
Synonyms : NET45
Summary: This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been identified, but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens dihydroxyacetone kinase 2 homolog (S. cerevisiae) (DAK) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens dihydroxyacetone kinase 2 homolog (S. cerevisiae) (DAK) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens dihydroxyacetone kinase 2 homolog (S. cerevisiae) (DAK) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of dihydroxyacetone kinase 2 homolog (S. cerevisiae) (DAK) Validated Antibodies • Rabbit polyclonal anti-DAK antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • DAK - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • DAK - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Dak - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • DAK - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Dak - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene DAK

If you would like OriGene Technologies to prepare a purified protein in the quantity of your own interest, simply fill out the form and click Submit. OriGene will contact you.

Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
* Quantity (µg)
Comments
* First Name
* Last Name
* Email Address
* Institution Name
* Country
* Phone