DCP1A (NM_018403) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC201330. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 63.1 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Transcription Factors Protein Pathways: RNA degradation

Protein Data

RefSeq: NP_060873	RefSeq Size: 2054	RefSeq ORF: 1749
LocusID: 55802	Cytogenetic: 3p21.1
Synonyms : HSA275986; Nbla00360; SMAD4IP1; SMIF
Summary: Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. [provided by RefSeq, Jul 2008].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A) as transfection-ready DNA • Lenti ORF clone of Human DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A), mGFP tagged • Lenti ORF clone of Human DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A) , Myc-DDK-tagged • GFP-tagged ORF clone of Homo sapiens DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A) Validated Antibodies • Purified Goat Polyclonal Antibody against DCP1A • Rabbit anti-Dcp1a polyclonal antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • DCP1A - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • DCP1A - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Dcp1a - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • DCP1A - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Dcp1a - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene DCP1A

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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