VAPA (NM_194434) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC201164. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 27.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Transmembrane Protein Pathways: Tight junction

Protein Data

RefSeq: NP_919415	RefSeq Size: 6859	RefSeq ORF: 750
LocusID: 9218	Cytogenetic: 18p11.22
Synonyms : hVAP-33; VAP-33; VAP-A; VAP33
Summary: The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa (VAPA), transcript variant 2 as transfection-ready DNA • Lenti ORF clone of Human VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa (VAPA), transcript variant 2, mGFP tagged • GFP-tagged ORF clone of Homo sapiens VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa (VAPA), transcript variant 2 as transfection-ready DNA • Lenti ORF clone of Human VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa (VAPA), transcript variant 2 , Myc-DDK-tagged • Myc-DDK-tagged ORF clone of Homo sapiens VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa (VAPA), transcript variant 2 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa (VAPA), transcript variant 2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • VAPA - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • VAPA - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Vapa - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • VAPA - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Vapa - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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