MAPKAPK3 (NM_004635) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200358. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 42.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. BioActivity: MAPKAPK3 activity verified in a biochemical assay: MAPKAPK3 (mitogen-activated protein kinase-activated protein kinase 3) (TP300358) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. MAPKAPK3 is a serine/threonine kinase that functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. Varying concentrations of MAPKAPK3 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors. Protein Families: Protein KinaseDruggable Genome Protein Pathways: MAPK signaling pathwayVEGF signaling pathway

Protein Data BioActivity Data

RefSeq: NP_004626	RefSeq Size: 2500	RefSeq ORF: 1149
LocusID: 7867	Cytogenetic: 3p21.3
Synonyms : 3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MK-3
Summary: This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • MAPKAPK3 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • MAPKAPK3 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Mapkapk3 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • MAPKAPK3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Mapkapk3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene MAPKAPK3

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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