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Home Recombinant Human Proteins All TP53 Proteins

TP53 (NM_000546) Purified Human Protein

Specifications Citations (2) Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP300003 Recombinant protein of human tumor protein p53 (TP53), transcript variant 1, 20 ug (Larger pack size?) $680 In Stock
UM500053 Purified P53 (TP53) mouse monoclonal antibody, clone UMAB62 $468 In Stock
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OriGene Data
Species:Human Expression Host:HEK293 cells
Expression cDNA Clone or AA Sequence
Recombinant protein was produced with TrueORF clone, RC200003. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag:C-terminal MYC/DDK Predicted MW:43.5 kDa
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>50 ug/mL as determined by microplate BCA method
Buffer:25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
BioActivity: TP53 Activity Verified in a DNA-binding Assay: TP53 (TP304649) activity was measured in a colorimetric DNA-binding assay. Double-stranded oligonucleotide containing the p53 consensus DNA-binding sequence was incubated with dilutions of the purified TP53 protein and TP53 bound to the oligo was captured onto the surface of a microtiter plate. After washing, bound TP53 was detected with an anti-p53 primary antibody followed by an HRP-labeled secondary antibody. After initial color development, the reaction was quenched and the color intensity was measured at 450nm.
Protein Families: Stem cell - PluripotencyTranscription FactorsDruggable Genome
Protein Pathways: MAPK signaling pathwayCell cyclep53 signaling pathwayApoptosisWnt signaling pathwayNeurotrophin signaling pathwayMore Pathways >>
Protein Data
Protein Image
BioActivity Data
BioActivity Image
 

Reference Data
RefSeq: NP_000537 RefSeq Size: 2591 RefSeq ORF: 1179
LocusID: 7157 Cytogenetic: 17p13.1  
Synonyms : BCC7; LFS1; P53; TRP53
Summary:
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

 

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