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Home cDNA Clone TrueORF All SPHK1 ORF Clones

SPHK1 (NM_182965) Human cDNA ORF Clone

Western Blot Validated

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC207086 SPHK1 (Myc-DDK-tagged)-Human sphingosine kinase 1 (SPHK1), transcript variant 2, 10µg
$540
In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC207086L2 Lenti-ORF clone of SPHK1 (mGFP-tagged)-Human sphingosine kinase 1 (SPHK1), transcript variant 2, (10ug)

$1000 3 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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TrueORF Data for RC207086
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1413 bp
Predicted Protein MW: 50.9 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Druggable Genome
Protein Pathways: Sphingolipid metabolismMetabolic pathwaysCalcium signaling pathwayVEGF signaling pathwayFc gamma R-mediated phagocytosis
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_182965.1, NP_892010
RefSeq Size: 2174 RefSeq ORF: 1413
Synonyms : SPHK
LocusID: 8877 Cytogenetic: 17q25.2
Summary: The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].

 

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