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Home cDNA Clone TrueORF All MYLK ORF Clones

MYLK (NM_053025) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC215976 MYLK (Myc-DDK-tagged)-Human myosin light chain kinase (MYLK), transcript variant 1, 10µg
$1880
In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in a GFP-tagging vector is also available

Cat. No. Description Vector Price Availability  
RG215976 MYLK (GFP-tagged) - Human myosin light chain kinase (MYLK), transcript variant 1, (10ug) 2070 8 Weeks
TA150041 2H8, Anti-tGFP monoclonal antibody, 100µl Datasheet $248 In Stock
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TrueORF Data for RC215976
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 5745 bp
Predicted Protein MW: 210.7 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Protein KinaseDruggable Genome
Protein Pathways: Calcium signaling pathwayVascular smooth muscle contractionFocal adhesionRegulation of actin cytoskeleton

Reference Data
RefSeq: NM_053025.3, NP_444253
RefSeq Size: 7852 RefSeq ORF: 5745
Synonyms : AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK1; smMLCK
LocusID: 4638 Cytogenetic: 3q21
Summary: This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008].

 

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