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 Home cDNA Clone TrueORF All B3GALT5 ORF Clones
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B3GALT5 (NM_006057) Human cDNA ORF Clone

Specifications Citations Clone of Other Species Product Documents
Cat. No. Description Price Availability  
RC215430 Myc-DDK-tagged ORF clone of Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 1 as transfection-ready DNA, 10µg
$460
 In Stock
RC515430 Myc-DDK-tagged ORF clone of Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 1 as 200 ug transfection-ready DNA $580 2~3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248
$198		 In Stock

$50 off anti-DDK and other anti-tag antibodies till June 30th, 2013

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC215430L1 Lenti ORF clone of Human UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 1 , Myc-DDK-tagged

$830 2 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $360 In Stock
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Also for B3GALT5 (NM_006057)
cDNA Clone shRNA/siRNA Lysate Protein Antibody Service
TrueORF Data for RC215430
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence 		ORF Size: 933 bp
Predicted Protein MW: 36 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Transmembrane
Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto seriesGlycosphingolipid biosynthesis - globo seriesMetabolic pathways
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_006057.1, NP_006048
 RefSeq Size: 2762 RefSeq ORF: 933
Synonyms : B3GalT-V; B3GalTx; B3T5; beta3Gal-T5; GLCT5
LocusID: 10317 Cytogenetic: 21q22.3
Summary: This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene encodes the most probable candidate for synthesis of the type 1 Lewis antigens which are frequently found to be elevated in gastrointestinal and pancreatic cancers. The encoded protein is inactive with N-linked glycoproteins and functions in mucin glycosylation. Alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2012].

 
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