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Home cDNA Clone TrueORF All CHKB ORF Clones
TrueORF Gold

CHKB (NM_005198) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC210253 CHKB (Myc-DDK-tagged)-Human choline kinase beta (CHKB), 10µg   
$560
In Stock
RG210253 CHKB (GFP-tagged) - Human choline kinase beta (CHKB), (10ug) $610 In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Cat. No. Description Price Availability
RC510253 0 2 weeks
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Clone Modification
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The same insert cloned in a GFP-tagging vector is also available

Cat. No. Description Vector Price Availability  
RG210253 CHKB (GFP-tagged) - Human choline kinase beta (CHKB), (10ug)

$610 In Stock
TA150041 2H8, Anti-tGFP monoclonal antibody, 100µl Datasheet $248 In Stock
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TrueORF Data for RC210253
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1188 bp
Predicted Protein MW: 45.1 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Druggable Genome
Protein Pathways: Glycerophospholipid metabolismMetabolic pathways
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq Explanation: NM_005198.3, NP_005189 RefSeq Size: 1595 RefSeq ORF: 1188
Synonyms : CHETK; CHKL; CK; CKB; CKEKB; EK; EKB; MDCMC
LocusID: 1120 Cytogenetic: 22q13.33 Domains: Choline_kinase
Gene Summary: Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009].

 

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