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Home cDNA Clone TrueORF All ETF1 ORF Clones
TrueORF Gold

ETF1 (NM_004730) Human cDNA ORF Clone

Specifications Citations (1) Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC220387 ETF1 (Myc-DDK-tagged)-Human eukaryotic translation termination factor 1 (ETF1), 10µg   
$550
In Stock
RG220387 ETF1 (GFP-tagged) - Human eukaryotic translation termination factor 1 (ETF1), (10ug) $600 In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Cat. No. Description Price Availability
RC520387 ETF1 (Myc-DDK-tagged)-Human eukaryotic translation termination factor 1 (ETF1), (200ug) $670 2 weeks
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The same insert cloned in a GFP-tagging vector is also available

Cat. No. Description Vector Price Availability  
RG220387 ETF1 (GFP-tagged) - Human eukaryotic translation termination factor 1 (ETF1), (10ug)

$600 In Stock
TA150041 2H8, Anti-tGFP monoclonal antibody, 100µl Datasheet $248 In Stock
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TrueORF Data for RC220387
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1314 bp
Predicted Protein MW: 48.9 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq Explanation: NM_004730.1, NP_004721 RefSeq Size: 3653 RefSeq ORF: 1314
Synonyms : D5S1995; ERF; ERF1; RF1; SUP45L1; TB3-1
LocusID: 2107 Cytogenetic: 5q31.1 Domains: eRF1_1, eRF1_2, eRF1_3
Gene Summary: This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X. [provided by RefSeq, Aug 2013].

 

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