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Home cDNA Clone TrueORF All PPP1R12A ORF Clones
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PPP1R12A (NM_002480) Human cDNA ORF Clone

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Cat. No. Description Price Availability  
RC223540 PPP1R12A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 1, 10µg   
$1530
In Stock
RG223540 PPP1R12A (GFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 1, (10ug) $1690 In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Cat. No. Description Price Availability
RC523540 PPP1R12A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 1, (200ug) $1660 2 weeks
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The same insert cloned in a GFP-tagging vector is also available

Cat. No. Description Vector Price Availability  
RG223540 PPP1R12A (GFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 1, (10ug)

$1690 In Stock
TA150041 2H8, Anti-tGFP monoclonal antibody, 100µl Datasheet $248 In Stock
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Also for PPP1R12A (NM_002480)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody
TrueORF Data for RC223540
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 3093 bp
Predicted Protein MW: 117 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Druggable Genome
Protein Pathways: Vascular smooth muscle contractionFocal adhesionLong-term potentiationRegulation of actin cytoskeleton
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq Explanation: NM_002480.1, NP_002471 RefSeq Size: 4613 RefSeq ORF: 3093
Synonyms : M130; MBS; MYPT1
LocusID: 4659 Cytogenetic: 12q15-q21 Domains: ANK
Gene Summary: Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009].

 

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