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Home cDNA Clone TrueORF All EN1 ORF Clones
TrueORF Gold

EN1 (NM_001426) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC224659 EN1 (Myc-DDK-tagged)-Human engrailed homeobox 1 (EN1), 10µg   
$480
In Stock
RG224659 EN1 (GFP-tagged) - Human engrailed homeobox 1 (EN1), (10ug) $540 In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Cat. No. Description Price Availability
RC524659 0 2 weeks
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Clone Modification
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The same insert cloned in Lenti and other vectors are also available.

Cat. No. Description Vector Price Availability  
RC224659L1 Lenti ORF clone of Human engrailed homeobox 1 (EN1) , Myc-DDK-tagged

$670 3 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
Lenti particle icon Add to Shopping Cart
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TrueORF Data for RC224659
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1179 bp
Predicted Protein MW: 39.9 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: ES Cell Differentiation/IPSDruggable Genome
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq Explanation: NM_001426.2, NP_001417 RefSeq Size: 2943 RefSeq ORF: 1179
Synonyms : OTTHUMP00000162091; engrailed homolog 1; engrailed homeobox 1
LocusID: 2019 Cytogenetic: 2q14.2
Gene Summary: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008].

 

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