Tryptophan 5 hydroxylase 2 (TPH2) (NM_173353) Human Tagged ORF Clone

CAT#: RC221193

TPH2 (Myc-DDK-tagged)-Human tryptophan hydroxylase 2 (TPH2)

Product Images

Circular map for RC221193

• 

  Coomassie blue staining of purified TPH2 protein (Cat# TP321193). The protein was produced from HEK293T cells transfected with TPH2 cDNA clone (Cat# RC221193) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tryptophan 5 hydroxylase 2
• Synonyms: ADHD7; NTPH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221193 representing NM_173353
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCAGCAATGATGATGTTTTCCAGTAAATACTGGGCACGGAGAGGGTTTTCCCTGGATTCAGCAG
  TGCCCGAAGAGCATCAGCTACTTGGCAGCTCAACACTAAATAAACCTAACTCTGGCAAAAATGACGACAA
  AGGCAACAAGGGAAGCAGCAAACGTGAAGCTGCTACCGAAAGTGGCAAGACAGCAGTTGTTTTCTCCTTG
  AAGAATGAAGTTGGTGGATTGGTAAAAGCACTGAGGCTCTTTCAGGAAAAACGTGTCAACATGGTTCATA
  TTGAATCCAGGAAATCTCGGCGAAGAAGTTCTGAGGTTGAAATCTTTGTGGACTGTGAGTGTGGGAAAAC
  AGAATTCAATGAGCTCATTCAGTTGCTGAAATTTCAAACCACTATTGTGACGCTGAATCCTCCAGAGAAC
  ATTTGGACAGAGGAAGAAGAGCTAGAGGATGTGCCCTGGTTCCCTCGGAAGATCTCTGAGTTAGACAAAT
  GCTCTCACAGAGTTCTCATGTATGGTTCTGAGCTTGATGCTGACCACCCAGGATTTAAGGACAATGTCTA
  TCGACAGAGAAGAAAGTATTTTGTGGATGTGGCCATGGGTTATAAATATGGTCAGCCCATTCCCAGGGTG
  GAGTATACTGAAGAAGAAACTAAAACTTGGGGTGTTGTATTCCGGGAGCTCTCCAAACTCTATCCCACTC
  ATGCTTGCCGAGAGTATTTGAAAAACTTCCCTCTGCTGACTAAATACTGTGGCTACAGAGAGGACAATGT
  GCCTCAACTCGAAGATGTCTCCATGTTTCTGAAAGAAAGGTCTGGCTTCACGGTGAGGCCGGTGGCTGGA
  TACCTGAGCCCACGAGACTTTCTGGCAGGACTGGCCTACAGAGTGTTCCACTGTACCCAGTACATCCGGC
  ATGGCTCAGATCCCCTCTACACCCCAGAACCAGACACATGCCATGAACTCTTGGGACATGTTCCACTACT
  TGCGGATCCTAAGTTTGCTCAGTTTTCACAAGAAATAGGTCTGGCGTCTCTGGGAGCATCAGATGAAGAT
  GTTCAGAAACTAGCCACGTGCTATTTCTTCACAATCGAGTTTGGCCTTTGCAAGCAAGAAGGGCAACTGC
  GGGCATATGGAGCAGGACTCCTTTCCTCCATTGGAGAATTAAAGCACGCCCTTTCTGACAAGGCATGTGT
  GAAAGCCTTTGACCCAAAGACAACTTGCTTACAGGAATGCCTTATCACCACCTTCCAGGAAGCCTACTTT
  GTTTCAGAAAGTTTTGAAGAAGCCAAAGAAAAGATGAGGGACTTTGCAAAGTCAATTACCCGTCCCTTCT
  CAGTATACTTCAATCCCTACACACAGAGTATTGAAATTCTGAAAGACACCAGAAGTATTGAAAATGTGGT
  GCAGGACCTTCGCAGCGACTTGAATACAGTGTGTGATGCTTTAAACAAAATGAACCAATATCTGGGGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221193 representing NM_173353
  Red=Cloning site Green=Tags(s)
  
  MQPAMMMFSSKYWARRGFSLDSAVPEEHQLLGSSTLNKPNSGKNDDKGNKGSSKREAATESGKTAVVFSL
  KNEVGGLVKALRLFQEKRVNMVHIESRKSRRRSSEVEIFVDCECGKTEFNELIQLLKFQTTIVTLNPPEN
  IWTEEEELEDVPWFPRKISELDKCSHRVLMYGSELDADHPGFKDNVYRQRRKYFVDVAMGYKYGQPIPRV
  EYTEEETKTWGVVFRELSKLYPTHACREYLKNFPLLTKYCGYREDNVPQLEDVSMFLKERSGFTVRPVAG
  YLSPRDFLAGLAYRVFHCTQYIRHGSDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDED
  VQKLATCYFFTIEFGLCKQEGQLRAYGAGLLSSIGELKHALSDKACVKAFDPKTTCLQECLITTFQEAYF
  VSESFEEAKEKMRDFAKSITRPFSVYFNPYTQSIEILKDTRSIENVVQDLRSDLNTVCDALNKMNQYLGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173353
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173353.4
• RefSeq Size: 2360 bp
• RefSeq ORF: 1473 bp
• Locus ID: 121278
• UniProt ID: Q8IWU9
• Cytogenetics: 12q21.1
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tryptophan metabolism
• MW: 56.1 kDa
• Gene Summary: This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. [provided by RefSeq, Feb 2016]