PPP2R3B (NM_013239) Human Tagged ORF Clone

CAT#: RC222908

PPP2R3B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B'', beta (PPP2R3B)

Product Images

Circular map for RC222908

• 

  Western blot validation of overexpression lysate (Cat# LY415717) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222908 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPP2R3B
• Synonyms: NYREN8; PPP2R3L; PPP2R3LY; PR48; PR70
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222908 representing NM_013239
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCCGGCAAAGTGCTGCAGCCGGTCCTGAAGATGAAGGTGGACGAGCTGTTCCTGTACTGGCTCA
  GCGAGGCCAGCACGCAGCGGATGCTGCAGGACTGCCTGCGCCGGATCAAGGCGCCCGGGCGGGACCAGCC
  GACCCCGGGGGACGGGGAGCAGCCCGGGGCCTGGCCCACAGCCCCGCTCGCCGCCCCCCGGCCCAGCGGG
  CTCGAACCCCCGGGAACCCCCGGGCCGGGCCCTGCGCTGCCCCTGGGCGCCGCCTCCAGCCCCAGGAACG
  CGCCCCACGTTCGAGGCACCCGTAGATCCGCAGGGACGAGAGTAGTTCAGACACGGAAAGAAGAGCCTCT
  GCCCCCGGCCACGAGCCAAAGCATTCCGACCTTCTACTTCCCCAGAGGACGCCCGCAGGACTCCGTCAAC
  GTGGATGCCGTCATCAGCAAGATCGAGAGCACCTTCGCCCGGTTCCCCCACGAGAGGGCCACCATGGATG
  ACATGGGCCTGGTGGCCAAGGCCTGCGGCTGCCCCCTCTACTGGAAGGGGCCGCTCTTCTATGGCGCCGG
  CGGGGAGCGCACGGGCTCCGTGTCCGTCCACAAGTTCGTCGCCATGTGGAGAAAAATCCTCCAGAACTGC
  CACGACGACGCGGCCAAGTTCGTCCATCTGCTCATGAGCCCCGGCTGCAACTACCTGGTGCAGGAGGACT
  TTGTCCCCTTCTTGCAGGACGTGGTGAACACGCACCCGGGGCTGTCGTTCCTGAAGGAGGCGTCCGAGTT
  CCACTCGCGCTACATCACCACGGTCATCCAGCGGATCTTCTACGCCGTGAACCGGTCCTGGTCCGGCAGG
  ATCACCTGCGCCGAGCTGCGGAGGAGCTCCTTCCTGCAGAATGTGGCGCTGCTGGAGGAGGAGGCGGACA
  TCAACCAGCTGACCGAATTCTTCTCGTACGAGCATTTCTACGTCATCTACTGCAAGTTCTGGGAGCTGGA
  CACGGACCACGACCTGCTCATCGACGCGGACGACCTGGCGCGGCACAATGACCACGCCCTTTCTACCAAG
  ATGATAGACAGGATCTTCTCAGGAGCAGTCACACGAGGCAGAAAAGTGCAGAAGGAAGGGAAGATCAGCT
  ATGCCGACTTTGTCTGGTTTTTGATCTCTGAGGAAGACAAAAAAACACCGACCAGCATCGAGTACTGGTT
  CCGCTGCATGGACCTGGACGGGGACGGCGCCCTGTCCATGTTCGAGCTCGAGTACTTCTACGAGGAGCAG
  TGCCGAAGGCTGGACAGCATGGCCATCGAGGCCCTGCCCTTCCAGGACTGCCTCTGCCAGATGCTGGACC
  TGGTCAAGCCGAGGACTGAAGGGAAGATCACGCTGCAGGACCTGAAGCGCTGCAAGCTGGCYAACGTCTT
  CTTCGACACCTTCTTCAACATCGAGAAGTACCTCGACCACGAGCAGAAAGAGCAGATCTCCCTGCTCAGG
  GACGGTGACAGCGGCGGCCCCGAGCTCTCGGACTGGGAGAAGTACGCGGCCGAGGAGTACGACATCCTGG
  TGGCCGAGGAGACYGYGGGAGAGCCCTGGGAGGACGGGTTCGAGGCCGAGCTCAGCCCYGTGGAGCAGAA
  GCTGAGTGCGCTGCGCTCCCCGCTGGCCCAGAGGCCCTTCTTCGAGGCGCCCTCACCGCTGGGCGCCGTG
  GACCTGTACGAGTACGCATGCGGGGACGAGGACCTGGAGCCGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222908 representing NM_013239
  Red=Cloning site Green=Tags(s)
  
  MPPGKVLQPVLKMKVDELFLYWLSEASTQRMLQDCLRRIKAPGRDQPTPGDGEQPGAWPTAPLAAPRPSG
  LEPPGTPGPGPALPLGAASSPRNAPHVRGTRRSAGTRVVQTRKEEPLPPATSQSIPTFYFPRGRPQDSVN
  VDAVISKIESTFARFPHERATMDDMGLVAKACGCPLYWKGPLFYGAGGERTGSVSVHKFVAMWRKILQNC
  HDDAAKFVHLLMSPGCNYLVQEDFVPFLQDVVNTHPGLSFLKEASEFHSRYITTVIQRIFYAVNRSWSGR
  ITCAELRRSSFLQNVALLEEEADINQLTEFFSYEHFYVIYCKFWELDTDHDLLIDADDLARHNDHALSTK
  MIDRIFSGAVTRGRKVQKEGKISYADFVWFLISEEDKKTPTSIEYWFRCMDLDGDGALSMFELEYFYEEQ
  CRRLDSMAIEALPFQDCLCQMLDLVKPRTEGKITLQDLKRCKLXNVFFDTFFNIEKYLDHEQKEQISLLR
  DGDSGGPELSDWEKYAAEEYDILVAEEXXGEPWEDGFEAELSXVEQKLSALRSPLAQRPFFEAPSPLGAV
  DLYEYACGDEDLEPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013239
• ORF Size: 1725 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013239.5
• RefSeq Size: 2071 bp
• RefSeq ORF: 1728 bp
• Locus ID: 28227
• UniProt ID: Q9Y5P8
• Cytogenetics: X;Y
• Protein Families: Druggable Genome, Phosphatase
• MW: 64.9 kDa
• Gene Summary: Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the beta subfamily of regulatory subunit B''. [provided by RefSeq, Apr 2010]