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OriGene cDNAs in recent publications

BAT3 and SET1A form a complex with CTCFL/BORIS to modulate H3K4 histone dimethylation and gene expressionMol. Cell. Biol. Nov.2008; 28(21): p. 6720-6729 [BAT3]


Heme Oxygenase-1 Regulates Cardiac Mitochondrial Biogenesis via Nrf2-Mediated Transcriptional Control of Nuclear Respiratory Factor-1, Circ. Res., Oct 2008; 10.1161/01.RES.0000338597.71702.ad. [AKT1]


Interactions of S100A2 and S100A6 with the Tetratricopeptide Repeat Proteins, Hsp90/Hsp70-organizing Protein and Kinesin Light ChainJ. Biol. Chem., Oct 2008; 283: 28246 - 28258. [TOR1A]


Significance of Organic Cation Transporter 3 (SLC22A3) Expression for the Cytotoxic Effect of Oxaliplatin in Colorectal Cancer, Drug Metab. Dispos., Nov 2008; 36: 2299 - 2306. [POU5F1(OCT3)]


Stroma-dependent apoptosis in clonal hematopoietic precursors correlates with expression of PYCARD Blood, Oct 2008; 10.1182/blood-2008-04-152686. [TNFRSF1A] 

37,000 tagged-TrueORF cDNA clones: by epitopes or by GFP

Express your proteins with epitope tags, GFP or in an inducible vector

Engineered as expression-ready clones with verified ORFs and built-in C-terminal tags, TrueORFs express proteins with tracking tags, allowing easy detection and isolation of expressed proteins. (Learn more..)

  • 25,000 human and 12,000 mouse clones
  • Three vectors for various applications:
    • pCMV6-Entry: C-terminal Myc-DDK* tag for detection and purification
    • pCMV6-AC-GFP: C-terminal GFP tag for visualization
    • pTUNE: Inducible expression
  • Many more tagging options through PrecisionShuttle
  • Sequence verified
  • Transfection-ready


GFP-tagged STAT3 moved into nucleus upon IL6 treatment



Search can be performed using any of the following terms: Gene Name, Gene Symbol, Accession Number or Keyword.

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Useful links:

TrueORF Entry Vector TrueORF GFP Vector TrueORF Inducible Vector Product Manual Destination vector

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Technical features or TrueORF:

  • The CMV promoter and a Kozak consensus sequence drive protein expression in mammalian cells.
  • The C-terminus of the ORF is tagged with Myc/DDK* or tGFP
  • Ten (10) ug of purified plasmid is provided for immediate transfection and expression experiments.
  • The upstream T7 promoter allows protein expression in cell-free system.
  • Engineered as the entry vector for the OriGene PrecisionShuttle system.

 

* Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C Flag® is a registered trademark of Sigma-Aldrich

 


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