Normal Mean and Range SerumSamples (observed):
Seventeen normal human plasma samples and 20 normal human plasma samples (not from the same donors) were tested in the Growth Factor Panel. The mean, median and range of the concentrations are tabulated in the table above (pg/ml).
Normal Mean and Range Plasmid Samples (observed):
Seventeen normal human plasma samples and 20 normal human plasma samples (not from the same donors) were tested in the Growth Factor Panel. The mean, median and range of the concentrations are tabulated in the table above (pg/ml).
Intra-assay CV – Serum/Plasma:
Four serum and four plasma samples were tested in replicates of six. Concentrations of analytes were calculated using Masterplex QT using a 5-parameter logistic regression with 1/Y weighting. CV values of greater than 20% correspond to samples whose analyte levels were close to the limit of detection.
Inter-assay CV – Serum/Plasma:
Nine serum and nine plasma samples were tested in the Growth Factor Panel on three separate days. For analytes with normal concentrations near the limit of detetion, samples were spiked with a range of analyte amounts. Concentrations of analytes were calculated using a five parameter logistic regression with 1/Y weighting. The average and standard deviation were calculated for each sample over three days.
Typical inter-assay reproducibility data for serum and plasma samples. Concentrations (pg/ml) of analytes were calculated using a five parameter logistic regression with 1/Y weighting. The average and standard deviation were calculated for each sample over three days.
Standard Curve Range:
Standard curve for PDGF-AB (One of the standard curves for the quantitation of 11 growth factors in a multiplexed ELISA assay using a color coded bead array and the Luminex xMAP® system.)
Standard curve for PDGF-BB (One of the standard curves for the quantitation of 11 growth factors in a multiplexed ELISA assay using a color coded bead array and the Luminex xMAP® system.)
Standard curve for FGF4 (One of the standard curves for the quantitation of 11 growth factors in a multiplexed ELISA assay using a color coded bead array and the Luminex xMAP® system.)
Standard curve for VEGFD (One of the standard curves for the quantitation of 11 growth factors in a multiplexed ELISA assay using a color coded bead array and the Luminex xMAP® system.)
Standard curve for FGF2 (One of the standard curves for the quantitation of 11 growth factors in a multiplexed ELISA assay using a color coded bead array and the Luminex xMAP® system.)
Standard curve for EGF (One of the standard curves for the quantitation of 11 growth factors in a multiplexed ELISA assay using a color coded bead array and the Luminex xMAP® system.)
Standard curve for HGF (One of the standard curves for the quantitation of 11 growth factors in a multiplexed ELISA assay using a color coded bead array and the Luminex xMAP® system.)
Standard curve for FLT3LG (One of the standard curves for the quantitation of 11 growth factors in a multiplexed ELISA assay using a color coded bead array and the Luminex xMAP® system.)
Standard curve for ANGPT2 (One of the standard curves for the quantitation of 11 growth factors in a multiplexed ELISA assay using a color coded bead array and the Luminex xMAP® system.)
Standard curve for PGF (One of the standard curves for the quantitation of 11 growth factors in a multiplexed ELISA assay using a color coded bead array and the Luminex xMAP® system.)
Standard curve for VEGFA (One of the standard curves for the quantitation of 11 growth factors in a multiplexed ELISA assay using a color coded bead array and the Luminex xMAP® system.)
Recovery:
Eight normal serum and eight normal plasma samples from different donors were each spiked with a fixed amount of recombinant protein standard. Spiked and un-spiked samples were then run in the the multiplexed Growth Factor Panel. Concentrations of analytes were calculated with Masterplex QT using a 5-parameter logistic regression with 1/Y weighting. The recovery was calculating by subtracting the concentration of the un-spiked sample from the concentration of the spiked sample and then dividing by the known amount spiked.
Linearity:
Four serum and four plasma samples were each spiked with a fixed amount of recombinant standard and then diluted 1:5, 1:10 and 1:20. Samples were run in the Growth Factor Panel and concentrations were calculated using a five parameter logistic regression with 1/Y weighting. Linearity was calculated by multiplying the pg/ml of the diluted samples by the dilution factor and expressing this result as a ratio to the undiluted sample.
