NANOG (NM_024865) Human Untagged Clone

CAT#: SC305169

NANOG (untagged)-Human Nanog homeobox (NANOG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NANOG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_024865 edited
  CGGCCGCGGAATCTTTTCCTCCTCTTCCTCTATACTAACATGAGTGTGGATCCAGCTTGT
  CCCCAAAGCTTGCCTTGCTTTGAAGCATCCGACTGTAAAGAATCTTCACCTATGCCTGTG
  ATTTGTGGGCCTGAAGAAAACTATCCATCCTTGCAAATGTCTTCTGCTGAGATGCCTCAC
  ACAGAGACTGTCTCTCCTCTTCCTTCCTCCATGGATCTGCTTATTCAGGACAGCCCTGAT
  TCTTCCACCAGTCCCAAAGGCAAACAACCCACTTCTGCAGAGAATAGTGTCGCAAAAAAG
  GAAGACAAGGTCCCGGTCAAGAAACAGAAGACCAGAACTGTGTTCTCTTCCACCCAGCTG
  TGTGTACTCAATGATAGATTTCAGAGACAGAAATACCTCAGCCTCCAGCAGATGCAAGAA
  CTCTCCAACATCCTGAACCTCAGCTACAAACAGGTGAAGACCTGGTTCCAGAACCAGAGA
  ATGAAATCTAAGAGGTGGCAGAAAAACAACTGGCCGAAGAATAGCAATGGTGTGACGCAG
  AAGGCCTCAGCACCTACCTACCCCAGCCTCTACTCTTCCTACCACCAGGGATGCCTGGTG
  AACCCGACTGGGAACCTTCCAATGTGGAGCAACCAGACCTGGAACAATTCAACCTGGAGC
  AACCAGACCCAGAACATCCAGTCCTGGAGCAACCACTCCTGGAACACTCAGACCTGGTGC
  ACCCAATCCTGGAACAATCAGGCCTGGAACAGTCCCTTCTATAACTGTGGAGAGGAATCT
  CTGCAGTCCTGCATGCACTTCCAGCCAAATTCTCCTGCCAGTGACTTGGAGGCTGCTTTG
  GAAGCTGCTGGGGAAGGCCTTAATGTAATACAGCAGACCACTAGGTATTTTAGTACTCCA
  CAAACCATGGATTTATTCCTAAACTACTCCATGAACATGCAACCTGAAGACGTGTGAAGA
  TGAGTGAAACTGATATTACTCAATTTCAGTCTGGACAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024865 unedited
  NNNGGAAGGTTCAGTATTGTATACGACTCATATAGGCGGCCGCGGAATCTTTTCCTCCTC
  TTCCTCTATACTAACATGAGTGTGGATCCAGCTTGTCCCCAAAGCTTGCCTTGCTTTGAA
  GCATCCGACTGTAAAGAATCTTCACCTATGCCTGTGATTTGTGGGCCTGAAGAAAACTAT
  CCATCCTTGCAAATGTCTTCTGCTGAGATGCCTCACACAGAGACTGTCTCTCCTCTTCCT
  TCCTCCATGGATCTGCTTATTCAGGACAGCCCTGATTCTTCCACCAGTCCCAAAGGCAAA
  CAACCCACTTCTGCAGAGAATAGTGTCGCAAAAAAGGAAGACAAGGTCCCGGTCAAGAAA
  CAGAAGACCAGAACTGTGTTCTCTTCCACCCAGCTGTGTGTACTCAATGATAGATTTCAG
  AGACAGAAATACCTCAGCCTCCAGCAGATGCAAGAACTCTCCAACATCCTGAACCTCAGC
  TACAAACAGGTGAAGACCTGGTTCCAGAACCAGAGAATGAAATCTAAGAGGTGGCAGAAA
  AACAACTGGCCGAAGAATAGCAATGGTGTGACGCAGAAGGCCTCAGCACCTACCTACCCC
  AGCCTCTACTCTTCCTACCACCAGGGATGCCTGGTGAACCCGACTGGGAACCTTCCAATG
  TGGAGCAACCAGACCTGGAACAATTCAACCTGGAGCAACCAGACCCAGAACATCCAGTCC
  TGGAGCAACCACTCCTGGAACACTCAGACCTGGTGCACCCAATCCTGGAACAATCANGCC
  TGGAACAGTCCCTTCTATAACTGTGGAGAGGAATCTCTGCAGTCCTGCATGCACTTCCAG
  CCAAATTCTCCTGCCAGTGACTTTGGAAGCTGCCTTTGGAGCTGCTGGGGGAAGGCCTTA
  TGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024865 unedited
  NGGGAATAGCTTGNNACCGCGGCCGCATNCTANGATCGAGCAGTGTCCAGACTGAAATTG
  AGTAATATCAGTTTCACTCATCTTCACACGTCTTCAGGTTGCATGTTCATGGAGTAGTTT
  AGGAATAAATCCATGGTTTGTGGAGTACTAAAATACCTAGTGGTCTGCTGTATTACATTA
  AGGCCTTCCCCAGCAGCTTCCAAGGCAGCCTCCAAGTCACTGGCAGGAGAATTTGGCTGG
  AAGTGCATGCAGGACTGCAGAGATTCCTCTCCACAGTTATAGAAGGGACTGTTCCAGGCC
  TGATTGTTCCAGGATTGGGTGCACCAGGTCTGAGTGTTCCAGGAGTGGTTGCTCCAGGAC
  TGGATGTTCTGGGTCTGGTTGCTCCAGGTTGAATTGTTCCAGGTCTGGTTGCTCCACATT
  GGAAGGTTCCCAGTCGGGTTCACCAGGCATCCCTGGTGGTAGGAAGAGTAGAGGCTGGGG
  TAGGTAGGTGCTGAGGCCTTCTGCGTCACACCATTGCTATTCTTCGGCCAGTTGTTTTTC
  TGCCACCTCTTAGATTTCATTCTCTGGTTCTGGAACCAGGTCTTCACCTGTTTGTAGCTG
  AGGTTCAGGATGTTGGAGAGTTCTTGCATCTGCTGGAGGCTGAGGTATTTCTGTCTCTGA
  AATCTATCATTGAGTACACACAGCTGGGTGGAAGAGAACACAGTTCTGGTCTTCTGTTTC
  TTGACCGGGACCTTGTCTTCCTTTTTTGCGACACTATTCTCTGCAGAAGTGGGTTGTTTG
  CCTTTGGGACTGGTGGAAGAATCANGGCTGTCCTGAATAAGCAGATCCATGNGAGAAAGG
  AAGAGAGAGACAGTCTCTGTGTGAGGCATCTCAGCAGAAGACATTTGCAAGGATGGATAG
  
• Restriction Sites: Please inquire
• ACCN: NM_024865
• Insert Size: 1200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: There are 5 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 1 amino acid. This substitution is validated by the human genome assemblies NT_010194.16 (NIH) and NW_925840.1 (Celera).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024865.1, NP_079141.1
• RefSeq Size: 2114 bp
• RefSeq ORF: 918 bp
• Locus ID: 79923
• UniProt ID: Q9H9S0
• Cytogenetics: 12p13.31
• Protein Families: Cancer stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Stem cell - Pluripotency
• Gene Summary: The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.