Gasdermin D (GSDMD) (NM_024736) Human Untagged Clone

CAT#: SC112028

GSDMD (untagged)-Human gasdermin D (GSDMD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Gasdermin D
• Synonyms: DF5L; DFNA5L; FKSG10; GSDMDC1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112028 sequence for NM_024736 edited (data generated by NextGen Sequencing)
  ATGGGGTCGGCCTTTGAGCGGGTAGTCCGGAGAGTGGTCCAGGAGCTGGACCATGGTGGG
  GAGTTCATCCCTGTGACCAGCCTGCAGAGCTCCACTGGCTTCCAGCCCTACTGCCTGGTG
  GTTAGGAAGCCCTCAAGCTCATGGTTCTGGAAACCCCGTTATAAGTGTGTCAACCTGTCT
  ATCAAGGACATCCTGGAGCCGGATGCCGCGGAACCAGACGTGCAGCGTGGCAGGAGCTTC
  CACTTCTACGATGCCATGGATGGGCAGATACAGGGCAGCGTGGAGCTGGCAGCCCCAGGA
  CAGGCAAAGATCGCAGGCGGGGCCGCGGTGTCTGACAGCTCCAGCACCTCAATGAATGTG
  TACTCGCTGAGTGTGGACCCTAACACCTGGCAGACTCTGCTCCATGAGAGGCACCTGCGG
  CAGCCAGAACACAAAGTCCTGCAGCAGCTGCGCAGCCGCGGGGACAACGTGTACGTGGTG
  ACTGAGGTGCTGCAGACACAGAAGGAGGTGGAAGTCACGCGCACCCACAAGCGGGAGGGC
  TCGGGCCGGTTTTCCCTGCCCGGAGCCACGTGCTTGCAGGGTGAGGGCCAGGGCCATCTG
  AGCCAGAAGAAGACGGTCACCATCCCCTCAGGCAGCACCCTCGCATTCCGGGTGGCCCAG
  CTGGTTATTGACTCTGACTTGGACGTCCTTCTCTTCCCGGATAAGAAGCAGAGGACCTTC
  CAGCCACCCGCGACAGGCCACAAGCGTTCCACGAGCGAAGGCGCCTGGCCACAGCTGCCC
  TCTGGCCTCTCCATGATGAGGTGCCTCCACAACTTCCTGACAGATGGGGTCCCTGCGGAG
  GGGGCGTTCACTGAAGACTTCCAGGGCCTACGGGCAGAGGTGGAGACCATCTCCAAGGAA
  CTGGAGCTTTTGGACAGAGAGCTGTGCCAGCTGCTGCTGGAGGGCCTGGAGGGGGTGCTG
  CGGGACCAGCTGGCCCTGCGAGCCTTGGAGGAGGCGCTGGAGCAGGGCCAGAGCCTTGGG
  CCGGTGGAGCCCCTGGACGGTCCAGCAGGTGCTGTCCTGGAGTGCCTGGTGTTGTCCTCC
  GGAATGCTGGTGCCGGAACTCGCTATCCCTGTTGTCTACCTGCTGGGGGCACTGACCATG
  CTGAGTGAAACGCAGCACAAGCTGCTGGCGGAGGCGCTGGAGTCGCAGACCCTGTTGGGG
  CCGCTCGAGCTGGTGGGCAGCCTCTTGGAGCAGAGTGCCCCGTGGCAGGAGCGCAGCACC
  ATGTCCCTGCCCCCCGGGCTCCTGGGGAACAGCTGGGGCGAAGGAGCACCGGCCTGGGTC
  TTGCTGGACGAGTGTGGCCTAGAGCTGGGGGAGGACACTCCCCACGTGTGCTGGGAGCCG
  CAGGCCCAGGGCCGCATGTGTGCACTCTACGCCTCCCTGGCACTGCTATCAGGACTGAGC
  CAGGAGCCCCACTAG
  
  Clone variation with respect to NM_024736.6
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024736 unedited
  TTCCGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCAGACG
  CGCCACCCTCGGGGCGCCGACGGTCACGGAGCATGGGGTCGGCCTTTGAGCGGGTAGTCC
  GGAGAGTGGTCCAGGAGCTGGACCATGGTGGGGAGTTCATCCCTGTGACCAGCCTGCAGA
  GCTCCACTGGCTTCCAGCCCTACTGCCTGGTGGTTAGGAAGCCCTCAAGCTCATGGTTCT
  GGAAACCCCGTTATAAGTGTGTCAACCTGTCTATCAAGGACATCCTGGAGCCGGATGCCG
  CGGAACCAGACGTGCAGCGTGGCAGGAGCTTCCACTTCTACGATGCCATGGATGGGCAGA
  TACAGGGCAGCGTGGAGCTGGCAGCCCCAGGACAGGCAAAGATCGCAGGCGGGGCCGCGG
  TGTCTGACAGCTCCAGCACCTCAATGAATGTGTACTCGCTGAGTGTGGACCCTAACACCT
  GGCAGACTCTGCTCCATGAGAGGCACCTGCGGCAGCCAGAACACAAAGTCCTGCAGCAGC
  TGCGCAGCCGCGGGGACAACGTGTACGTGGTGACTGAGGTGCTGCAGACACAGAAGGAGG
  TGGAAGTCACGCGCACCCACAAGCGGGAGGGCTCGGGCCGGTTTTCCCTGCCCGGAGCCA
  CGTGCTTGCAGGGTGAGGGCCAGGGCCATCTGAGCCAGAAGAAGACGGTCACCATCCCCT
  CAGGCAGCACCCTCGCATTCCGGGTGGCCCAGCTGGTTATTGACTCTGACTTGNACGTCC
  TTCTCTTCCCGGATAAGAGCAGAGGACCTTCCAGCCACCCGCGACAGGCCACAGCGTTCA
  CGAGCGAAGCGCCTGGCCCAGCTGCCTCTGGCTCTCATGATGAGGTGCCTCACACTTCTG
  AAGAGGGGTCCTGCGAGGGGCGTTCCTGAGACTTNCAGGCTACGGCAGAGTGGAACATCT
  CCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024736 unedited
  CTATGGACCGCGCCGCAATTTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTCCTTCGTATGCCACCTTTATTGGGTTTCCCCAACTCCTGGGCCCCATGG
  TAAACTGGCCACATGGCTACTGGGCTCCTGGCCTTCCTAGGGCTAGCAGCTGGGGGGCAA
  ACACTCTGCCCTGCTGGAAAGCTGCCAGGCCATGCCCGGGCACAGGCTAGTGGGGCTCCT
  GGCTCAGTCCTGATAGCAGTGCCAGGGAGGCGTAAAGTGCACACATGCGGCCCTGGGCCT
  GCGGCTCCCAGCACACGTGGGGAGTGTCCTCCCCCAGCTTTAGGCCACACTCGTCCAGCA
  AGACCCAGGCCGGTGCTCCTTCGCCCCAGCTGTTCCCCAGGAGCCCGGGGGGCAGGGACA
  TGGTGCTGCGCTCCTGCCACGGGGCACTCTGCTCCAAGAGGCTGCCCACCAGCTCGAGCG
  GCCCCAACAGGGTCTGCGACTCCAGCGCCTCCGCCAGCAGCTTGTGCTGCGTTTCACTCA
  GCATGGTCAGTGCCCCCAGCAGGTAGACAACAGGGATAGCGAGTTCCGGCACCAGCATTC
  CGGAGGACAACACCAGGCACTCCAGGACAGCACCTGCTGGACCGTCCAGGGGCTCCACCG
  GCCCAAGGCTCTGGCCCTGGCTCAGCGCCTCCTCCAAGCTCGCAGGGCCAGCTGGTCCCG
  CAGCACCCCCTTCAGGCCCCTCAGCAGCAGCTGGCAAAGCTTCTGTCCAAAAGCTCCAGT
  TCCTTTGGAGATGGTCTCCACCTCTGCCCGTAGGCCCTGNAAGTCTCAATGAACGCCCCC
  TCCGCAGGGACCCATCTGTCAGNAAGTTGTGGAAGCACCTTCTCATGGAAAGCCCAAGGG
  CANCTGTGGCCAGGCCCCTCCCTCGTGGAACCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024736
• Insert Size: 1750 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024736.3, NP_079012.1
• RefSeq Size: 1823 bp
• RefSeq ORF: 1455 bp
• Locus ID: 79792
• UniProt ID: P57764
• Cytogenetics: 8q24.3
• Domains: DFNA5
• Gene Summary: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein.