Apc2 (ANAPC2) (NM_013366) Human Untagged Clone

CAT#: SC115268

ANAPC2 (untagged)-Human anaphase promoting complex subunit 2 (ANAPC2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Apc2
• Synonyms: APC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115268 sequence for NM_013366 edited (data generated by NextGen Sequencing)
  ATGGCGGCGGCAGTTGTGGTGGCGGAGGGGGACAGCGACTCCCGGCCCGGACAGGAGTTG
  TTAGTGGCCTGGAACACCGTGAGCACCGGCCTGGTGCCGCCGGCTGCGCTGGGGCTGGTG
  TCTTCCCGGACCAGCGGTGCAGTCCCGCCAAAGGAAGAGGAGCTCCGGGCGGCGGTGGAG
  GTTCTGAGGGGCCACGGGCTACACTCGGTCCTGGAGGAGTGGTTCGTGGAGGTGCTGCAG
  AACGATCTGCAGGCCAACATCTCCCCTGAGTTCTGGAATGCCATCTCCCAATGCGAGAAC
  TCTGCGGATGAGCCCCAGTGCCTTTTGCTACTCCTTGACGCTTTTGGCCTGCTGGAGAGC
  CGCCTGGATCCCTACCTGCGTAGCCTAGAGCTGCTGGAGAAATGGACTCGCCTGGGCTTG
  CTGATGGGCACTGGTGCTCAGGGGCTGCGAGAAGAAGTCCACACTATGTTGCGCGGAGTC
  TTGTTCTTTAGCACCCCCAGAACCTTCCAAGAGATGATCCAGCGTCTGTATGGGTGCTTC
  TTGAGAGTCTATATGCAGAGTAAGAGGAAGGGGGAAGGGGGCACAGACCCGGAACTGGAA
  GGGGAGCTGGACAGCCGGTATGCCCGTCGCCGGTACTACCGGCTCCTGCAGAGCCCGCTG
  TGTGCAGGGTGCAGCAGTGACAAGCAACAGTGCTGGTGTCGCCAGGCTCTGGAGCAGTTC
  CATCAGCTCAGCCAGGTCTTACACAGGCTCAGTCTGCTGGAGCGGGTCAGTGCCGAGGCT
  GTGACCACCACCCTGCACCAGGTGACCCGGGAGAGGATGGAGGACCGTTGCCGGGGCGAG
  TACGAGCGCTCCTTCCTGCGTGAGTTCCACAAGTGGATCGAGCGGGTGGTCGGCTGGCTC
  GGCAAGGTGTTCCTGCAGGACGGCCCCGCCAGGCCCGCATCTCCCGAGGCCGGCAACACC
  CTGCGCCGCTGGCGCTGCCACGTGCAAAGGTTCTTCTACCGCATCTACGCCAGCCTGCGC
  ATCGAGGAGCTCTTCAGCATCGTCCGAGACTTCCCAGACTCCCGGCCAGCCATCGAGGAC
  CTCAAGTACTGCCTGGAGAGGACGGACCAGAGGCAGCAGCTGCTCGTGTCCCTCAAGGCT
  GCCCTGGAGACTCGGCTCCTGCATCCAGGCGTCAACACGTGTGACATCATCACCCTCTAT
  ATCTCTGCCATCAAGGCGCTGCGCGTGCTGGACCCTTCCATGGTCATCCTGGAGGTGGCC
  TGTGAGCCTATCCGCCGCTACCTGAGGACGCGGGAGGACACAGTGCGGCAGATTGTGGCT
  GGGCTGACGGGGGACTCGGACGGGACAGGGGACCTGGCTGTTGAGCTGTCCAAGACCGAC
  CCGGCGAGCCTGGAGACAGGCCAGGACAGTGAGGATGACTCAGGCGAGCCAGAGGACTGG
  GTCCCGGACCCTGTGGATGCCGATCCAGGGAAGTCGAGCTCCAAGCGGCGTTCATCGGAC
  ATCATCAGCCTGCTGGTCAGCATCTACGGCAGCAAGGACCTCTTCATCAATGAGTACCGC
  TCGCTGCTGGCCGACCGCCTGCTGCACCAGTTCAGCTTCAGCCCCGAGCGGGAGATCCGC
  AACGTGGAGCTGCTGAAGCTGCGCTTTGGCGAGGCCCCAATGCACTTCTGTGAAGTCATG
  CTGAAGGACATGGCGGACTCCCGCCGCATCAATGCCAACATCCGGGAGGAGGATGAGAAG
  CGGCCAGCAGAGGAGCAGCCACCGTTCGGGGTCTACGCTGTCATCCTGTCCAGTGAGTTC
  TGGCCGCCCTTCAAGGACGAGAAGCTGGAGGTCCCCGAGGATATCAGGGCAGCCCTGGAG
  GCTTACTGCAAGAAGTATGAGCAGCTCAAGGCCATGCGGACCCTCAGTTGGAAGCACACC
  CTGGGCCTGGTGACCATGGACGTGGAGCTGGCCGACCGCACGCTGTCTGTGGCGGTCACC
  CCAGTACAGGCGGTGATCTTGCTGTATTTTCAGGACCAAGCCAGCTGGACCCTGGAGGAA
  CTGAGCAAGGCGGTGAAGATGCCCGTGGCGCTGCTGCGGCGGCGGATGTCCGTGTGGCTG
  CAGCAGGGTGTGCTGCGTGAGGAGCCCCCCGGCACCTTCTCTGTCATTGAGGAGGAGCGG
  CCTCAGGACCGGGACAACATGGTGCTCATTGACAGTGACGACGAGAGCGACTCCGGCATG
  GCCTCCCAGGCCGACCAGAAGGAGGAGGAGCTGCTGCTCTTCTGGACGTACATCCAGGCC
  ATGCTGACCAACCTGGAGAGCCTCTCACTGGATCGTATCTACAACATGCTCCGCATGTTT
  GTGGTGACTGGGCCTGCACTGGCCGAGATTGACCTGCAGGAGCTGCAGGGCTACCTGCAG
  AAGAAGGTGCGGGACCAGCAGCTCGTCTACTCGGCCGGCGTCTACCGCCTGCCCAAGAAC
  TGCAGCTGA
  
  Clone variation with respect to NM_013366.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013366 unedited
  TTGAGGGATTTTGGAATACGATCTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCCG
  AGCGAATCTCGGAGTCGGTGGGTGCAGATGGCGGCGGCAGTTGTGGTGGCGGAGGGGGAC
  AGCGACTCCCGGCCCGGACAGGAGTTGTTAGTGGCCTGGAACACCGTGAGCACCGGCCTG
  GTGCCGCCGGCTGCGCTGGGGCTGGTGTCTTCCCGGACCAGCGGTGCAGTCCCGCCAAAG
  GAAGAGGAGCTCCGGGCGGCGGTGGAGGTTCTGAGGGGCCACGGGCTACACTCGGTCCTG
  GAGGAGTGGTTCGTGGAGGTGCTGCAGAACGATCTGCAGGCCAACATCTCCCCTGAGTTC
  TGGAATGCCATCTCCCAATGCGAGAACTCTGCGGATGAGCCCCAGTGCCTTTTGCTACTC
  CTTGACGCTTTTGGCCTGCTGGAGAGCCGCCTGGATCCCTACCTGCGTAGCCTAGAGCTG
  CTGGAGAAATGGACTCGCCTGGGCTTGCTGATGGGCACTGGTGCTCAGGGGCTGCGAGAA
  GAAGTCCACACTATGTTGCTCGGAGTCTTGTTCTTTAGCACCCCCAGAACCTTCCAAGAG
  ATGATCCAGCGTCTGTATGGGTGCTTCTTGAGAGTCTATATGCAGAGTAAGAGGAAGGGG
  GAAGGGGGCACAGACCCGGGACTGGAAGGGGAGCTGGACCAGCCGTTTGCCCGTCGCCCG
  TACTACCGACTCCTGCAGAGCCCGCTGTGTGCAGGCTGCCACATTGACCAACCACAGTGC
  TGGGTGTCGCCAGCTCTGGAGCAGTTCCTCAGCTTAACCAGGTCTTACACAGGCTAATTC
  TGCTGGAACAGCTCAATGCCGCAGCTTGGACCCCACCCTTGGACCAGTGACCCGGTGAAA
  GGATGAAGGAACCTTTGCCGGGGCGAGATCCAACCTTCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013366 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGATGATCTGACTTGCTTTAATCT
  GCACACTGCGGGGGTGGGGGTGGGCATGCTCCTCAGCAGTGCATTCTGGGACACGGGCGG
  GCGGGGGCTGGCACGGGAGGACGAGAGCACCTGCAGGGCAGCGCCTGGCGGGCGGGCGGG
  CGGGCGGGCGATGTGTCAGCTGCAGTTCTTGGGCAGGCGGTAGACGCCGGCCGAGTAGAC
  GAGCTGCTGGTCCCGCACCTTCTTCTGCAGGTAGCCCTGCAGCTCCTGCAGGTCAATCTC
  GGCCAGTGCAGGCCCAGTCACCACAAACATGCGGAGCATGTTGTAGATACGATCCAGTGA
  GAGGCTCTCCAGGTTGGTCAGCATGGCCTGGATGTACGTCCAGAAGAGCAGCAGCTCCTC
  CTCCTTCTGGTCGGCCTGGGAGGCCATGCCGGAGTCGCTCTCGTCGTCACTGTCAATGAG
  CACCATGTTGTCCCGGTCCTGAGGCCGCTCCTCCTCAATGACAGAGAAGGTGCCGGGGGG
  CTCCTCACGCAGCACACCCTGCTGCAGCCACACGGACATCCGCCGCCGCAGCAGCGCCAC
  GGGCATCTTCACCGCCTTGCTCAGTTCCTCCAGGGTCCAGCTGGCTTGGTCCTGAAAATA
  CAGCAAGATCACCGCCTGTACTGGGGTGACCGCACAGACAGCGTGCGGTCGGCCAGCTCC
  ACGTCCATGTCCACAGGCCCAGGTGTGCTTCCAACTGAGTCCGCATGCCCTGAGCTGCTA
  TACTTCTGCAGTAGCCTCAGGCCTGCCTGAATCCTGGGGACTCCACTTTCGTCCTGAGGC
  CGGCCNACTACTTCCAGAGACAGGTAACCCCAACGTGGTGTCCTTGTTGCCGGTTATCTT
  CTCGGATGTGCTTGAGCGCGGAGTCCCAGTCTTAATGATTCACAAGCCTGGCTCCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013366
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013366.3, NP_037498.1
• RefSeq Size: 2733 bp
• RefSeq ORF: 2469 bp
• Locus ID: 29882
• UniProt ID: Q9UJX6
• Cytogenetics: 9q34.3
• Domains: CULLIN
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• Gene Summary: A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq, Jul 2008]