PI 3 Kinase catalytic subunit alpha (PIK3CA) (NM_006218) Human Untagged Clone

CAT#: SC116227

PIK3CA (untagged)-Human phosphoinositide-3-kinase, catalytic, alpha polypeptide (PIK3CA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PI 3 Kinase catalytic subunit alpha
• Synonyms: CLAPO; CLOVE; CWS5; MCAP; MCM; MCMTC; p110-alpha; PI3K; PI3K-alpha
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116227 sequence for NM_006218 edited (data generated by NextGen Sequencing)
  
  
  ATGCCTCCACGACCATCATCAGGTGAACTGTGGGGCATCCACTTGATGCCCCCAAGAATCCTAGTAGAAT
  GTTTACTACCAAATGGAATGATAGTGACTTTAGAATGCCTCCGTGAGGCTACATTAATAACCATAAAGCA
  TGAACTATTTAAAGAAGCAAGAAAATACCCCCTCCATCAACTTCTTCAAGATGAATCTTCTTACATTTTC
  GTAAGTGTTACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTGTGACCTTCGGC
  TTTTTCAACCCTTTTTAAAAGTAATTGAACCAGTAGGCAACCGTGAAGAAAAGATCCTCAATCGAGAAAT
  TGGTTTTGCTATCGGCATGCCAGTGTGTGAATTTGATATGGTTAAAGATCCAGAAGTACAGGACTTCCGA
  AGAAATATTCTGAACGTTTGTAAAGAAGCTGTGGATCTTAGGGACCTCAATTCACCTCATAGTAGAGCAA
  TGTATGTCTATCCTCCAAATGTAGAATCTTCACCAGAATTGCCAAAGCACATATATAATAAATTAGATAA
  AGGGCAAATAATAGTGGTGATCTGGGTAATAGTTTCTCCAAATAATGACAAGCAGAAGTATACTCTGAAA
  ATCAACCATGACTGTGTACCAGAACAAGTAATTGCTGAAGCAATCAGGAAAAAAACTCGAAGTATGTTGC
  TATCCTCTGAACAACTAAAACTCTGTGTTTTAGAATATCAGGGCAAGTATATTTTAAAAGTGTGTGGATG
  TGATGAATACTTCCTAGAAAAATATCCTCTGAGTCAGTATAAGTATATAAGAAGCTGTATAATGCTTGGG
  AGGATGCCCAATTTGATGTTGATGGCTAAAGAAAGCCTTTATTCTCAACTGCCAATGGACTGTTTTACAA
  TGCCATCTTATTCCAGACGCATTTCCACAGCTACACCATATATGAATGGAGAAACATCTACAAAATCCCT
  TTGGGTTATAAATAGTGCACTCAGAATAAAAATTCTTTGTGCAACCTACGTGAATGTAAATATTCGAGAC
  ATTGATAAGATCTATGTTCGAACAGGTATCTACCATGGAGGAGAACCCTTATGTGACAATGTGAACACTC
  AAAGAGTACCTTGTTCCAATCCCAGGTGGAATGAATGGCTGAATTATGATATATACATTCCTGATCTTCC
  TCGTGCTGCTCGACTTTGCCTTTCCATTTGCTCTGTTAAAGGCCGAAAGGGTGCTAAAGAGGAACACTGT
  CCATTGGCATGGGGAAATATAAACTTGTTTGATTACACAGACACTCTAGTATCTGGAAAAATGGCTTTGA
  ATCTTTGGCCAGTACCTCATGGATTAGAAGATTTGCTGAACCCTATTGGTGTTACTGGATCAAATCCAAA
  TAAAGAAACTCCATGCTTAGAGTTGGAGTTTGACTGGTTCAGCAGTGTGGTAAAGTTCCCAGATATGTCA
  GTGATTGAAGAGCATGCCAATTGGTCTGTATCCCGAGAAGCAGGATTTAGCTATTCCCACGCAGGACTGA
  GTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACG
  AGATCCTCTCTCTGAAATCACTGAGCAGGAGAAAGATTTTCTATGGAGTCACAGACACTATTGTGTAACT
  ATCCCCGAAATTCTACCCAAATTGCTTCTGTCTGTTAAATGGAATTCTAGAGATGAAGTAGCCCAGATGT
  ATTGCTTGGTAAAAGATTGGCCTCCAATCAAACCTGAACAGGCTATGGAACTTCTGGACTGTAATTACCC
  AGATCCTATGGTTCGAGGTTTTGCTGTTCGGTGCTTGGAAAAATATTTAACAGATGACAAACTTTCTCAG
  TATTTAATTCAGCTAGTACAGGTCCTAAAATATGAACAATATTTGGATAACTTGCTTGTGAGATTTTTAC
  TGAAGAAAGCATTGACTAATCAAAGGATTGGGCACTTTTTCTTTTGGCATTTAAAATCTGAGATGCACAA
  TAAAACAGTTAGCCAGAGGTTTGGCCTGCTTTTGGAGTCCTATTGTCGTGCATGTGGGATGTATTTGAAG
  CACCTGAATAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCAAACAGGAGAAGA
  AGGATGAAACACAAAAGGTACAGATGAAGTTTTTAGTTGAGCAAATGAGGCGACCAGATTTCATGGATGC
  TCTACAGGGCTTTCTGTCTCCTCTAAACCCTGCTCATCAACTAGGAAACCTCAGGCTTGAAGAGTGTCGA
  ATTATGTCCTCTGCAAAAAGGCCACTGTGGTTGAATTGGGAGAACCCAGACATCATGTCAGAGTTACTGT
  TTCAGAACAATGAGATCATCTTTAAAAATGGGGATGATTTACGGCAAGATATGCTAACACTTCAAATTAT
  TCGTATTATGGAAAATATCTGGCAAAATCAAGGTCTTGATCTTCGAATGTTACCTTATGGTTGTCTGTCA
  ATCGGTGACTGTGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACTATTATGCAAATTCAGTGCAAAG
  GCGGCTTGAAAGGTGCACTGCAGTTCAACAGCCACACACTACATCAGTGGCTCAAAGACAAGAACAAAGG
  AGAAATATATGATGCAGCCATTGACCTGTTTACACGTTCATGTGCTGGATACTGTGTAGCTACCTTCATT
  TTGGGAATTGGAGATCGTCACAATAGTAACATCATGGTGAAAGACGATGGACAACTGTTTCATATAGATT
  TTGGACACTTTTTGGATCACAAGAAGAAAAAATTTGGTTATAAACGAGAACGTGTGCCATTTGTTTTGAC
  ACAGGATTTCTTAATAGTGATTAGTAAAGGAGCCCAAGAATGCACAAAGACAAGAGAATTTGAGAGGTTT
  CAGGAGATGTGTTACAAGGCTTATCTAGCTATTCGACAGCATGCCAATCTCTTCATAAATCTTTTCTCAA
  TGATGCTTGGCTCTGGAATGCCAGAACTACAATCTTTTGATGACATTGCATACATTCGAAAGACCCTAGC
  CTTAGATAAAACTGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATGGTGGC
  TGGACAACAAAAATGGATTGGATCTTCCACACAATTAAACAGCATGCATTGAACTGA
  
  Clone variation with respect to NM_006218.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006218 unedited
  NCCTGTTCAAATTTGTATCCGACTCACTATAGGCGGCCGCGCAATTCGCACGAGGAGGGA
  GCGCACAAGAGGTCTTTCTAGAAACAGTCTGCATATTCTTTAATGAATTAGAGTTGTGAT
  TTCATCTTGGTGAAGCTTCCTCATTCCAGCACTGCGCCTTTGTGCACATGCAGGACCTGG
  GTTTTCGGAACGATGTTTTGAATCATAGTTTGTGTGGCCGGGGAGAACCCCTTAGAATAC
  TTGGGTTTCTGCTTTGGGACAACCATACATCTAATTCCTTAAAGTAGTTTTATATGTAAA
  ACTTGCAAAGAATCAGAACAATGCCTCCACGACCATCATCAGGTGAACTGTGGGGCATCC
  ACTTGATGCCCCCAAGAATCCTAGTAGAATGTTTACTACCAAATGGAATGATAGTGACTT
  TAGAATGCCTCCGTGAGGCTACATTAATAACCATAAAGCATGAACTATTTAAAGAAGCAA
  GAAAATACCCCCTCCATCAACTTCTTCAAGATGAATCTTCTTACATTTTCGTAAGTGTTA
  CTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTGTGACCTTCGGC
  TTTTTCAACCCTTTTTAAAAGTAATTGAACCAGTAGGCAACCGTGAAGAAAAGATCCTCA
  ATCGAGAAATTGGTTTTGCTATCGGCATGCCAGTGTGTGAATTTGATATGGTTAAAGATC
  CAGAAGTACAGGACTTTCGAAGAATATTCTGAACGTTTGTAAAGAAGCTGTGGATCTTTT
  AGGACCTCATTCACCTCATAGTAGAGCAATGTATGTCTATCCTCCAATGTAGAATCTTCA
  CCAGAATGCCAAAGCCATATTTTATAATAGATAAAGGCAATN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006218 unedited
  ACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTACTTTGTGGGAGCCCA
  GAATTTCCCTGGGAAATAAGAATAAGATCCTTTAAATACTGTCCAGAAGAAAAAAAAAAA
  TACCACCTTATAACAGGTCTCATTTGTGACTGCTTCCAAAACTGCAGATAAAACCTCAAA
  TCATCAAAAGGTTTGATTTATTAAAAAGAAAAAGTCTCTGGTGAAATCTCAAGAGTCCCT
  TCCACCCCAAGCATTTTTCTTCATTAAACAAGCAGTCAAAAACCCACTTCAAACTATAAA
  TTGAACAGCAATGGAAAAATAAGAAATTTTCATTCTTTTCCAATCAATGTAATTTAACAT
  AAACTCCAGTTTACTTACACCTAATGCAATAACAGCCTTTGTTGTGTCCACATTTCAAAA
  CATAGAACATTTGCAAGACAAAAAAACCATCACTTTTTCCTTCTCCATCATTTCTATATA
  TTTTGGGGATTTTTGTTTTGTTTTGTTTTTTAAAAAAAGATACAAACAATCTTCAAAGTT
  TACCTTTTTGGACTTAAGGCATAACATGAAATTGCGCATTATTCTAAAGCTTAATTTTGA
  AATGAACTAGTTTAAGTGCTATCAAACCCTGTTTGCGTTTACATTATTTAAATTATATAG
  NATTTTATTTCTGTTCTTGCTGNAAATCTAATGCTGTTCATGGATTGTGCAATTCCTATG
  CNATCGGTCTTTGCCTGCTGAGAGTTATTAACAGNTGCAGTGTGGAATCCAGAGTGAGCT
  TTCATTTTCTCAGNTATCTTTTTCAGTCATGCATGCTGTTTAATGTGTGGAAGATCCATC
  CATTTTGNTGTCCAGCACATGATGTGCTCATTCATTNGNTATGAATACTNCAAAGCCTCT
  GCTCAGTTTACTAGGCTAAGGTCTTCAATGATGCATGCATAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006218
• Insert Size: 4530 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006218.2, NP_006209.2
• RefSeq Size: 9104 bp
• RefSeq ORF: 3207 bp
• Locus ID: 5290
• UniProt ID: P42336
• Cytogenetics: 3q26.32
• Domains: PI3K_rbd, PI3_PI4_kinase, PI3Ka, PI3K_C2, PI3K_p85B
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Inositol phosphate metabolism, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway
• Gene Summary: Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016]