LGI1 (NM_005097) Human Untagged Clone

CAT#: SC116925

LGI1 (untagged)-Human leucine-rich, glioma inactivated 1 (LGI1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LGI1
• Synonyms: ADLTE; ADPAEF; ADPEAF; EPITEMPIN; EPT; ETL1; IB1099
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116925 sequence for NM_005097 edited (data generated by NextGen Sequencing)
  ATGGAATCAGAAAGAAGCAAAAGGATGGGAAATGCCTGCATTCCCCTGAAAAGAATTGCT
  TATTTCCTATGTCTCTTATCTGCGCTTTTGCTGACTGAGGGGAAGAAACCAGCGAAGCCA
  AAATGCCCTGCCGTGTGTACTTGTACCAAAGATAATGCTTTATGTGAGAATGCCAGATCC
  ATTCCACGCACCGTTCCTCCTGATGTTATCTCATTATCCTTTGTGAGATCTGGTTTTACT
  GAAATCTCAGAAGGGAGTTTTTTATTCACGCCATCGCTGCAGCTCTTGTTATTCACATCG
  AACTCCTTTGATGTGATCAGTGATGATGCTTTTATTGGTCTTCCACATCTAGAGTATTTA
  TTCATAGAAAACAACAACATCAAGTCAATTTCAAGACATACTTTCCGGGGACTAAAGTCA
  TTAATTCACTTGAGCCTTGCAAACAACAATCTCCAGACACTCCCAAAAGATATTTTCAAA
  GGCCTGGATTCTTTAACAAATGTGGACCTGAGGGGTAATTCATTTAATTGTGACTGTAAA
  CTGAAATGGCTAGTGGAATGGCTTGGCCACACCAATGCAACTGTTGAAGACATCTACTGC
  GAAGGCCCCCCAGAATACAAGAAGCGCAAAATCAATAGTCTCTCCTCGAAGGATTTCGAT
  TGCATCATTACAGAATTTGCAAAGTCTCAAGACCTGCCTTATCAATCATTGTCCATAGAC
  ACTTTTTCTTATTTGAATGATGAGTATGTAGTCATCGCTCAGCCTTTTACTGGAAAATGC
  ATTTTCCTTGAATGGGACCATGTGGAAAAGACCTTCCGGAATTATGACAACATTACAGGC
  ACATCCACTGTAGTATGCAAGCCTATAGTCATTGAAACTCAGCTCTATGTTATTGTGGCC
  CAGCTGTTTGGTGGCTCTCACATCTATAAGCGAGACAGTTTTGCAAATAAATTCATAAAA
  ATCCAGGATATTGAAATTCTCAAAATCCGAAAACCCAATGACATTGAAACATTCAAGATT
  GAAAACAACTGGTACTTTGTTGTTGCTGACAGTTCAAAAGCTGGTTTTACTACCATTTAC
  AAATGGAACGGAAACGGATTCTACTCCCATCAATCCTTACACGCGTGGTACAGGGACACT
  GATGTGGAATATCTAGAAATAGTCAGAACACCTCAGACACTCAGAACGCCTCATTTAATT
  CTGTCTAGTAGTTCCCAGCGTCCTGTAATTTATCAGTGGAACAAAGCAACACAATTATTC
  ACTAACCAAACTGACATTCCTAACATGGAGGATGTGTACGCAGTGAAGCACTTCTCAGTG
  AAAGGGGACGTGTACATTTGCTTGACAAGATTCATTGGTGATTCCAAAGTCATGAAATGG
  GGAGGCTCCTCGTTCCAGGATATTCAGAGGATGCCATCGCGAGGATCCATGGTGTTCCAG
  CCTCTTCAAATAAATAATTACCAATATGCAATTCTTGGAAGTGATTACTCCTTTACTCAA
  GTGTATAACTGGGATGCAGAGAAAGCCAAATTTGTGAAATTTCAGGAATTAAATGTTCAG
  GCACCAAGATCATTCACACATGTGTCCATTAATAAGCGTAATTTTCTTTTTGCTTCCAGT
  TTTAAGGGAAATACACAGATTTACAAACATGTCATAGTTGACTTAAGCGCATGA
  
  Clone variation with respect to NM_005097.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005097 unedited
  NGGTTCANGATATTTGTATACGACTCACTATAGNNGGCGGCCGCGCAATTCGGCACGAGG
  GAGCAGTGCATTGCTGGAGCGAGGAGAAGCTCACGAATCAGCTGCAGGTCTCTGTTTTGA
  AAAAGCAGAGATACAGAGGCAGAGGAAAAGGGTGGACTCCTATGTGACCTGTTCTTAGAG
  CAAGACAATCACCATCTGAATTCCAGAAGCCCTGTTCATGGTTGGGGATATTTTCTCGAC
  TGCATGGAATCAGAAAGAAGCAAAAGGATGGGAAATGCCTGCATTCCCCTGAAAAGAATT
  GCTTATTTCCTATGTCTCTTATCTGCGCTTTTGCTGACTGAGGGGAAGAAACCAGCGAAG
  CCAAAATGCCCTGCCGTGTGTACTTGTACCAAAGATAATGCTTTATGTGAGAATGCCAGA
  TCCATTCCACGCACCGTTCCTCCTGATGTTATCTCATTATCCTTTGTGAGATCTGGTTTT
  ACTGAAATCTCAGAAGGGAGTTTTTTATTCACGCCATCGCTGCAGCTCTTGTTATTCACA
  TCGAACTCCTTTGATGTGATCAGTGATGATGCTTTTATTGGTCTTCCACATCTAGAGTAT
  TTATTCATAGAAAACAACAACATCAAGTCAATTTCAAGACATACTTTCCGGGGACTAAAG
  TCATTAATTCACTTGAGCCTTGCAAACAACAATCTCCAGACACTCCCAAAAGAATTTTCA
  AAGGCCTGGATTCTTTAACANATGTGGACCTGAGGGGTAATTCATTTATTGTGACTGGTA
  ACTGAAATGGCTAGTGGAATGGCTTGGCCACACCAATGCACTGTTGAAGACATCTACTGC
  GAAGGCCCCCCAGATTACAGAGCGCAAATCATAGTCTCTCCTCGAAGATTTCGATTGCTC
  ATTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005097 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAAAAAAATGTAAACAT
  TTTATTTGGCAGTTGTTTCTGTACACATGAATAAATAAATGGAAAAGTACATATTAATAT
  TTCTTTTTGGAGTAACAGATACCATTAAACGCTTATTTACACATCTTCACTGCAAAGAAC
  AGTTACACAATTAAATTTTGTCTTATAAAAGGTAACTTCCCACATCACTGGACTGGACAG
  TTAAAGGATGAATAACTGGTAAGGCTTGGTTTTAACAACCAAAAGTGTGGAAGGGGGTTT
  TTTTTTGCGGGGGGGAAAAAAAAAAAAAACCCCCTTTTTGTTGTTTTTCCCGGGGGGTTT
  TTGGGAAAAAAAAAATTTGTGGGGGGCGCCCACAAAAGAGGGGGGGGGGGCCCCCCCCCC
  CAAAAAAAAAAAAAAGGGGTGGGGGGGNGGNTTTTTTTTTTTTTTTTTCTTTAAAAAGGG
  GGGAGAAAAAAAAAAAANNCCTTTTTTTTTTTTTTCTCCCGCGGGGGGGGGGGGGGGTGT
  CTTCCCCCCCCCCCCCCCTCTCTTATAATACAAAAACNNNTTTGTTTGGTTTTTTTTTTT
  TCCCCCCCCGCCCCCGCGGGGGCGGGGGGGGGGGAGGGAGAAAAACACCCATCTCATTNN
  TTTCTTTTTTTTTTTTTTTTTTTAGGAAAATAAGGGGGNGGGGGGGNNGCCCCCCCCNCT
  CTCTCTTCNTTCTTTCCTCTCTCCTGTATCTCATACCCCTNCCCAACTCCCNCCATTANN
  NNTATATGTTGNGGGAATGCAAAAGGGACTGCCGGGNGCTTCCTGCCCTCCGTTCGTNCC
  TCCTCTCCTCCCATGCCCTTTTCACCGTAGTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005097
• Insert Size: 2240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005097.1, NP_005088.1
• RefSeq Size: 2254 bp
• RefSeq ORF: 1674 bp
• Locus ID: 9211
• UniProt ID: O95970
• Cytogenetics: 10q23.33
• Domains: LRRCT, LRR, LRR_TYP, EPTP
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: This gene encodes a member of the secreted leucine-rich repeat (LRR) superfamily and shares homology with members of the SLIT protein family. The encoded protein may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. This gene is rearranged as a result of translocations in glioblastoma cell lines, and it is frequently down-regulated or rearranged in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).