Beta Arrestin 2 (ARRB2) (NM_004313) Human Untagged Clone

CAT#: SC108950

ARRB2 (untagged)-Human arrestin, beta 2 (ARRB2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Beta Arrestin 2
• Synonyms: ARB2; ARR2; BARR2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004313 edited
  GAATTCGGCACGAGGTGGCAGCGGGCGAGGAGGCTGCGAGCGAGCCGCGAACCGAGCGGG
  CGGCGGGCGCGCGCACCATGGGGGAGAAACCCGGGACCAGGGTCTTCAAGAAGTCGAGCC
  CTAACTGCAAGCTCACCGTGTACTTGGGCAAGCGGGACTTCGTAGATCACCTGGACAAAG
  TGGACCCTGTAGATGGCGTGGTGCTTGTGGACCCTGACTACCTGAAGGACCGCAAAGTGT
  TTGTGACCCTCACCTGCGCCTTCCGCTATGGCCGTGAAGACCTGGATGTGCTGGGCTTGT
  CCTTCCGCAAAGACCTGTTCATCGCCACCTACCAGGCCTTCCCCCCGGTGCCCAACCCAC
  CCCGGCCCCCCACCCGCCTGCAGGACCGGCTGCTGAGGAAGCTGGGCCAGCATGCCCACC
  CCTTCTTCTTCACCATACCCCAGAATCTTCCATGCTCCGTCACACTGCAGCCAGGCCCAG
  AGGATACAGGAAAGGCCTGCGGCGTAGACTTTGAGATTCGAGCCTTCTGTGCTAAATCAC
  TAGAAGAGAAAAGCCACAAAAGGAACTCTGTGCGGCTGGTGATCCGAAAGGTGCAGTTCG
  CCCCGGAGAAACCCGGCCCCCAGCCTTCAGCCGAAACCACACGCCACTTCCTCATGTCTG
  ACCGGTCCCTGCACCTCGAGGCTTCCCTGGACAAGGAGCTGTACTACCATGGGGAGCCCC
  TCAATGTAAATGTCCACGTCACCAACAACTCCACCAAGACCGTCAAGAAGATCAAAGTCT
  CTGTGAGACAGTACGCCGACATCTGCCTCTTCAGCACCGCCCAGTACAAGTGTCCTGTGG
  CTCAACTCGAACAAGATGACCAGGTATCTCCCAGCTCCACATTCTGTAAGGTGTACACCA
  TAACCCCACTGCTCAGTGACAACCGGGAGAAGCGGGGTCTCGCCCTGGATGGGAAACTCA
  AGCACGAGGACACCAACCTGGCTTCCAGCACCATCGTGAAGGAGGGTGCCAACAAGGAGG
  TGCTGGGAATCCTGGTGTCCTACAGGGTCAAGGTGAAGCTGGTGGTGTCTCGAGGCGGGG
  ATGTCTCTGTGGAGCTGCCTTTTGTTCTTATGCACCCCAAGCCCCACGACCACATCCCCC
  TCCCCAGACCCCAGTCAGCCGCTCCGGAGACAGATGTCCCTGTGGACACCAACCTCATTG
  AATTTGATACCAACTATGCCACAGATGATGACATTGTGTTTGAGGACTTTGCCCGGCTTC
  GGCTGAAGGGGATGAAGGATGACGACTATGATGATCAACTCTGCTAGGAAGCGGGGTGGG
  AAGAAGGGAGGGGATGGGGTTGGGAGAGGTGAGGGCAGGATTAAGATCCCCACTGTCAAT
  GGGGGATTGTCCCAGCCCCTCTTCCCTTCCCCTCACCTGGAAGCTTCTTCAACCAATCCC
  TTCACACTCTCTCCCCCATCCCCCCAAGATACACACTGGACCCTCTCTTGCTGAATGTGG
  GCATTAATTTTTTGACTGCAGCTCTGCTTCTCCAGCCCCGCCGTGGGTGGCAAGCTGTGT
  TCATACCTAAATTTTCTGGAAGGGGACAGTGAAAAGAGGAGTGACAGGAGGGAAAGGGGG
  AGACAAAACTCCTACTCTCAACCTCACACCAACACCTCCCATTATCACTCTCTCTGCCCC
  CATTCCTTCAAGAGGAGACCCTTTGGGGACAAGGCCGTTTCTTTGTTTCTGAGCATAAAG
  AAGAAAATAAATCTTTTACTAAGAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004313 unedited
  TGTCAGAAATTTTGTATACGAACTCACTATAGGGCGGCCGCGATTCGGCACGAGGTGGCA
  GCGGGCGAGGAGGCTGCGAGCGAGCCGCGAACCGAGCGGGCGGCGGGCGCGCGCACCATG
  GGGGAGAAACCCGGGACCAGGGTCTTCAAGAAGTCGAGCCCTAACTGCAAGCTCACCGTG
  TACTTGGGCAAGCGGGACTTCGTAGATCACCTGGACAAAGTGGACCCTGTAGATGGCGTG
  GTGCTTGTGGACCCTGACTACCTGAAGGACCGCAAAGTGTTTGTGACCCTCACCTGCGCC
  TTCCGCTATGGCCGTGAAGACCTGGATGTGCTGGGCTTGTCCTTCCGCAAAGACCTGTTC
  ATCGCCACCTAACCAGGCCTTCCCCCCCGGTGCCCAACCCACCCCGGCCCCCCACCCGCC
  TGCAGGACCGGCTGCTGAGGAAGCTGGGCCAGCATGCCCACCCCTTCTTCTTCACCATAC
  CCCAGAATCTTCCATGCTCCGTCACACTGCAGCCAGGCCCAGAGGATACAGGAAAGGCCT
  GCGGCGTAGACTTTGAGATTCGAGCCTTCTGTGCTAAATCACTAGAAGAGAAAAGCCACA
  AAAGGAACTCTGTGCGGCTGGTGATCCGAAAGGTGCAGTTCGCCCCGGAGAAACCCGGCC
  CCCAGCCTTCAGCCGAAACCACACGCCACTTCCTCATGTCTGACCGGTCCCTGCACCTCG
  AGGCTTCCCTGGACAGGGAGCTGTACTACCATGGNGAGCCCCTCATGTANATGTCCACGT
  CACCAACAACTCCACCAAGACCGTNCAGAAGATCANAGTCTCTGTGAGACAGTACGCCGA
  CATCTGCCTCTTCAGCACCGCCCAGTACAAGTGTCCTGTGGCTCAACTCGAACAGAATGA
  CCAGTATCTCCCCAGCTCCATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004313 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTCTTAGTAAAAGATTTATTTTTC
  TTCTTTATGCTCAGAAACAAAGAAACGGCCTTGTCCCCAAAGGGTCTCCTCTTGAAGGAA
  TGGGGGCAGAGAGAGTGATAATGGGAGGTGTTGGTGTGAGGTTGAAAGTAGGAGTTTTGT
  CTCCCCCTTTCCCTCCTGTCACTCCTCTTTTCACTGTCCCCTTCCAGAAAATTTAGGTAT
  GAACACAGCTTGCCACCCACGGCGGGGCTGGAGAAGCAGAGCTGCAGTCAAAAAATTAAT
  GCCCACATTCAGCAAGAGAGGGTCCAGTGTGTATCTTGGGGGGATGGGGGAGAGAGTGTG
  AAGGGATCTGGTTTGAAGAAGCTTCCAGGTGAGGGGAAGGGAAGAGGGGCTGGGACAATC
  CCCCATTGACAGTGGGGATCTTAATCCTGCCCTCACCTCTCCCAACCCCATCCCCTCCCT
  TCTTCCCACCCCGCTTCCTAGCAGAGTTGATCATCATAGTCGTCATCCTTCATCCCCTTC
  AGCCGAAGCCGGGCAAAGTCCTCAAACACAATGTCATCATCTGTGGCATAGTTGGTATCA
  AATTCAATGAGGTTTGGTGTCCACAGGGACATCTGTCTCCGGAGCGGCTGACTGGGGTCT
  GAGGATGGGGNATGCTGGTCNTGGGGGCTGGGGGTGCATAANAACAAAGGCATCTGCCAG
  AGACATCCCCGCCTCGAGACACACAAGTTTAACCTAGACCCTGTAAGACACCAGGATTCC
  CAGCACTTCCTTGNTGGCACCCTNCTCACGATGGTGCTGGAAGCCAGGTTGGTGTCCTCG
  AGCTTGAGTTTGCATTCAGGGCGATACCCCGCTCTCCCGGTTGCACTGGCCATGGGGGTT
  GGGGGTCCCCTACAAATTGGGAGCGGGAATCCCGGCATCTTGTCAATTGACCCAGGCCTT
  GTCCTGGCGGGCGAAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004313
• Insert Size: 1880 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004313.3, NP_004304.1
• RefSeq Size: 1936 bp
• RefSeq ORF: 1230 bp
• Locus ID: 409
• UniProt ID: P32121
• Cytogenetics: 17p13.2
• Domains: arrestin
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Endocytosis, MAPK signaling pathway, Olfactory transduction
• Gene Summary: Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (1) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 3. The resulting isoform (1) lacks an alternate internal segment compared to isoform 3.