AFM (NM_001133) Human Untagged Clone

CAT#: SC119416

AFM (untagged)-Human afamin (AFM)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AFM
• Synonyms: ALB2; ALBA; ALF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119416 sequence for NM_001133 edited (data generated by NextGen Sequencing)
  ATGAAACTACTAAAACTTACAGGTTTTATTTTTTTCTTGTTTTTTTTGACTGAATCCCTA
  ACCCTGCCCACACAACCTCGGGATATAGAGAACTTCAATAGTACTCAAAAATTTATAGAA
  GATAATATTGAATACATCACCATCATTGCATTTGCTCAGTATGTTCAGGAAGCAACCTTT
  GAAGAAATGGAAAAGCTGGTGAAAGACATGGTAGAATACAAAGACAGATGTATGGCTGAC
  AAGACGCTCCCAGAGTGTTCAAAATTACCTAATAATGTTTTACAGGAAAAAATATGTGCT
  ATGGAGGGGCTGCCACAAAAGCATAATTTCTCACACTGCTGCAGTAAGGTTGATGCTCAA
  AGAAGACTCTGTTTCTTCTATAACAAGAAATCTGATGTGGGATTTCTGCCTCCTTTCCCT
  ACCCTGGATCCCGAAGAGAAATGCCAGGCTTATGAAAGTAACAGAGAATCCCTTTTAAAT
  CACTTTTTATATGAAGTTGCCAGAAGGAACCCATTTGTCTTCGCCCCTACACTTCTAACT
  GTTGCTGTTCATTTTGAGGAGGTGGCCAAATCATGTTGTGAAGAACAAAACAAAGTCAAC
  TGCCTTCAAACAAGGGCAATACCTGTCACACAATATTTAAAAGCATTTTCTTCTTATCAA
  AAACATGTCTGTGGGGCACTTTTGAAATTTGGAACCAAAGTTGTACACTTTATATATATT
  GCGATACTCAGTCAAAAATTCCCCAAGATTGAATTTAAGGAGCTTATTTCTCTTGTAGAA
  GATGTTTCTTCCAACTATGATGGATGCTGTGAAGGGGATGTTGTGCAGTGCATCCGTGAC
  ACGAGCAAGGTTATGAACCATATTTGTTCAAAACAAGATTCTATCTCCAGCAAAATCAAA
  GAGTGCTGTGAAAAGAAAATACCAGAGCGCGGCCAGTGCATAATTAACTCAAACAAAGAT
  GATAGACCAAAGGATTTATCTCTAAGAGAAGGAAAATTTACTGACAGTGAAAATGTGTGT
  CAAGAACGAGATGCTGACCCAGACACCTTCTTTGCGAAGTTTACTTTTGAATACTCAAGG
  AGACATCCAGACCTGTCTATACCAGAGCTTTTAAGAATTGTTCAAATATACAAAGATCTC
  CTGAGAAATTGCTGCAACACAGAAAACCCTCCAGGTTGTTACCGTTACGCGGAAGACAAA
  TTCAATGAGACAACTGAGAAAAGCCTCAAGATGGTACAACAAGAATGTAAACATTTCCAG
  AATTTGGGGAAGGATGGTTTGAAATACCATTACCTCATTAGGCTCACGAAGATAGCTCCC
  CAACTCTCCACTGAAGAACTGGTGTCTCTTGGCGAGAAAATGGTGACAGCTTTCACTACT
  TGCTGTACGCTAAGTGAAGAGTTTGCCTGTGTTGATAATTTGGCAGATTTAGTTTTTGGA
  GAGTTATGTGGAGTAAATGAAAATCGAACTATCAACCCTGCTGTGGACCACTGCTGTAAA
  ACAAACTTTGCCTTCAGAAGGCCCTGCTTTGAGAGTTTGAAAGCTGATAAAACATATGTG
  CCTCCACCTTTCTCTCAAGATTTATTTACCTTTCACGCAGACATGTGTCAATCTCAGAAT
  GAGGAGCTTCAGAGGAAGACAGACAGGTTTCTTGTCAACTTAGTGAAGCTGAAGCATGAA
  CTCACAGATGAAGAGCTGCAGTCTTTGTTTACAAATTTCGCAAATGTAGTGGATAAGTGC
  TGCAAAGCAGAGAGTCCTGAAGTCTGCTTTAATGAAGAGAGTCCAAAAATTGGCAACTGA
  
  Clone variation with respect to NM_001133.2
  1299 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001133 unedited
  NGGCGGTTCAGAATTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACCAGCAAAA
  ATGAAACTACTAAAACTTACAGGTTTTATTTTTTTCTTGTTTTTTTTGACTGAATCCCTA
  ACCCTGCCCACACAACCTCGGGATATAGAGAACTTCAATAGTACTCAAAAATTTATAGAA
  GATAATATTGAATACATCACCATCATTGCATTTGCTCAGTATGTTCAGGAAGCAACCTTT
  GAAGAAATGGAAAAGCTGGTGAAAGACATGGTAGAATACAAAGACAGATGTATGGCTGAC
  AAGACGCTCCCAGAGTGTTCAAAATTACCTAATAATGTTTTACAGGAAAAAATATGTGCT
  ATGGAGGGGCTGCCACAAAAGCATAATTTCTCACACTGCTGCAGTAAGGTTGATGCTCAA
  AGAAGACTCTGTTTCTTCTATAACAAGAAATCTGATGTGGGATTTCTGCCTCCTTTCCCT
  ACCCTGGATCCCGAAGAGAAATGCCAGGCTTATGAAAGTAACAGAGAATCCCTTTTAAAT
  CACTTTTTATATGAAGTTGCCAGAAGGAACCCATTTGTCTTCGCCCCTACACTTCTAACT
  GTTGCTGTTCATTTTGAGGAGGTGGCCAAATCATGTTGTGAAGAACAAAACAAAGTCAAC
  TGCCTTCAAACAAGGGCAATACCTGTCACACAATATTTAAAAGCATTTTCTTCTTATCAA
  AACATGTCTGTGGGGCACTTTTGAAATTTGGAACCAAAGTTGTACACTTTATATATATTG
  CGATACTCAGTCAAAAATTCCCCAAGATTGAATNTAAGGGAGCTATTTNCTCTTGTANAA
  GATGTTTCTTCCACTATGATGNATGCTGTGAAGGGGGAATGTTGTGCANNTGCATTCCGT
  GACACGAGCAGGNTAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001133 unedited
  TTTGCCCCAAATTACGTTGNACCGGCCCGTATACTAGATCGAGTTTTCTTTTTTTTTTTT
  TTTTTTTTTTTTTTTAACTTTACAAAAGTTGTGTTTATTGCTTGCTGCAATGTATTATTT
  CAGGTGACAGTTACAGAAAAATCCTTTTATTTTGTTCTCAGGAAATGCGATTCATCACCA
  CACAGATAGGAAGCCTTCCCTTTGGTGCTTTTTTTTTTACATATCTCCAGCAGCTGGCTT
  CAGTTGCCAATTTTTGGACTCTCTTCATTAAAGCAGACTTCAGGACTCTCTGCTTTGCAG
  CACTTATCCACTACATTTGCGAAATTTGTAAACAAAGACTGCAGCTCTTCATCTGTGAGT
  TCATGCTTCAGCTTCACTAAGTTGACAAGAAACCTGTCTGTCTTCCTCTGAAGCTCCTCA
  TTCTGAGATTGACACATGTCTGCGTGAAAGGTAAATAAATCTTGAGAGAAAGGTGGAGGC
  ACATATGTTTTATCAGCTTTCAAACTCTCAAAGCAGGGCCTTCTGAAGGCAAAGTTTGTT
  TTACAGCAGTGGTCCACAGCAGGGTTGATAGTTCGATTTTCATTTACTCCACATAACTCT
  CCAAAAACTAAATCTGCCAAATTATCAACACAGGCAAACTCTTCACTTAGCGTACAGCAA
  GTAGTGAAAGCTGTCACCATTTTCTCGCCAAGAGACACCAGTTCTTCAGTGGAGAGTTGG
  GGAGCTATCTTCGTGAGCCTAATGAGGTAATGGTATTTCAAACCATCCTTCCCCAAATTC
  TGGAAATGTTTACATTCTTGTTGTACCATCTTGAGGCTTTTCTCAGTTGCCTCAATGAAT
  TTGTCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001133
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001133.2, NP_001124.1
• RefSeq Size: 1997 bp
• RefSeq ORF: 1800 bp
• Locus ID: 173
• UniProt ID: P43652
• Cytogenetics: 4q13.3
• Domains: ALBUMIN
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq, Jul 2008]