GABA A Receptor alpha 1 (GABRA1) (NM_000806) Human Untagged Clone

CAT#: SC119668

GABRA1 (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GABA A Receptor alpha 1
• Synonyms: DEE19; ECA4; EIEE19; EJM; EJM5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119668 sequence for NM_000806 edited (data generated by NextGen Sequencing)
  ATGAGGAAAAGTCCAGGTCTGTCTGACTGTCTTTGGGCCTGGATCCTCCTTCTGAGCACA
  CTGACTGGAAGAAGCTATGGACAGCCGTCATTACAAGATGAACTTAAAGACAATACCACT
  GTCTTCACCAGGATTTTGGACAGACTCCTAGATGGTTATGACAATCGCCTGAGACCAGGA
  TTGGGAGAGCGTGTAACCGAAGTGAAGACTGATATCTTCGTCACCAGTTTCGGACCCGTT
  TCAGACCATGATATGGAATATACAATAGATGTATTTTTCCGTCAAAGCTGGAAGGATGAA
  AGGTTAAAATTTAAAGGACCTATGACAGTCCTCCGGTTAAATAACCTAATGGCAAGTAAA
  ATCTGGACTCCGGACACATTTTTCCACAATGGAAAGAAGTCAGTGGCCCACAACATGACC
  ATGCCCAACAAACTCCTGCGGATCACAGAGGATGGCACCTTGCTGTACACCATGAGGCTG
  ACAGTGAGAGCTGAATGTCCGATGCATTTGGAGGACTTCCCTATGGATGCCCATGCTTGC
  CCACTAAAATTTGGAAGTTATGCTTATACAAGAGCAGAAGTTGTTTATGAATGGACCAGA
  GAGCCAGCACGCTCAGTGGTTGTAGCAGAAGATGGATCACGTCTAAACCAGTATGACCTT
  CTTGGACAAACAGTAGACTCTGGAATTGTCCAGTCAAGTACAGGAGAATATGTTGTTATG
  ACCACTCATTTCCACTTGAAGAGAAAGATTGGCTACTTTGTTATTCAAACATACCTGCCA
  TGCATAATGACAGTGATTCTCTCACAAGTCTCCTTCTGGCTCAACAGAGAGTCTGTACCA
  GCAAGAACTGTCTTTGGAGTAACAACTGTGCTCACCATGACAACATTGAGCATCAGTGCC
  AGAAACTCCCTCCCTAAGGTGGCTTATGCAACAGCTATGGATTGGTTTATTGCCGTGTGC
  TATGCCTTTGTGTTCTCAGCTCTGATTGAGTTTGCCACAGTAAACTATTTCACTAAGAGA
  GGTTATGCATGGGATGGCAAAAGTGTGGTTCCAGAAAAGCCAAAGAAAGTAAAGGATCCT
  CTTATTAAGAAAAACAACACTTACGCTCCAACAGCAACCAGCTACACCCCTAATTTGGCC
  AGGGGCGACCCGGGCTTAGCCACCATTGCTAAAAGTGCAACCATAGAACCTAAAGAGGTC
  AAGCCCGAAACAAAACCACCAGAACCCAAGAAAACCTTTAACAGTGTCAGCAAAATTGAC
  CGACTGTCAAGAATAGCCTTCCCGCTGCTATTTGGAATCTTTAACTTAGTCTACTGGGCT
  ACGTATTTAAACAGAGAGCCTCAGCTAAAAGCCCCCACACCACATCAATAG
  
  Clone variation with respect to NM_000806.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000806 unedited
  TGTAAACGCTCCTCAGGGCGGCCGCGACTTCGGCACGAGGCTGACTTCTAAAAATCCTCC
  TCTCCCTCTCCCTTTTCCTAATCCGAGAATGATGGAGCTCGAGGCAAAGGAATGATTCCG
  GAAATGGAGATATGATTCTCAAACCTAGAAAATGATCGGAGTGATTTATTAGTTAAATAT
  TCTTCGTCCAGGAACCCAGCACAATTCAGAGCTGCAGATTGGATATTGGGAAGCAAATTT
  GGGTGTGAAATCTTCAGCAAAGGAGCACGCAGAGTCCATGATGGCTCAGACCAAGTGAGT
  GAGAGGCAGAGCGAGGACGCCCCTCTGCTCTGGCGCGCCCGGACTCGGACTCGCAGACTC
  GTGCTGGCTCCAGTCTCTCCACGATTCTCTCTCCCAGACTTTTCCCCGGTCTTAAGAGAT
  CCTGTGTCCAGAGGGGGCCTTAGCTGCTCCAGCCCGCGATGAGGAAAAGTCCAGGTCTGT
  CTGACTGTCTTTGGGCCTGGATCCTCCTTCTGAGCACACTGACTGGAAGAAGCTATGGAC
  AGCCGTCATTACAAGATGAACTTAAAGACAATACCACTGTCTTCACCAGGATTTTGGACA
  GACTCCTAGATGGTTATGACAATCGCCTGAGACCAGGATTGGGAAGAGCGTGTAACCGAA
  GTGAAGACTGATATTCTTCGTCACCAGTTTCGGGACCCGTTTTCAGACCATGATATGGGA
  TATACAATAGATGTATTTTTTTCCGTCAAGCTGGAAGGATGGAAGGTNTAAAATTAAAGG
  GACTTATGAACGGTCCCTCCGGTAAAATAACCTATGGGCAAGAAAATCTGGGACTTCGAC
  ACATTTTTTTCCATGGAAAGAAATAATGGGCCACAACATGACCATGCCCAAAAATCCTGG
  GGATAAAAGAAAAGGACCTTTTGTGACACATGAAGGGGAGAGAAAAAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000806 unedited
  GGCAACCTTCCAGGGCCAGGNANAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT
  TTTCTTGGATCTTTGAACCATCTTCCCCCTCTTTTCTCTCTCATTCTGTCTCCTTCTGAC
  ATGACTTTGCTCTTTCGCTTGCTCTGTCAAAATCCTGTCTCCAAGCTGCATAGTCTGCTT
  TGCTCCAAACTGCCAGACAGGGGTCTCTTGTTCTTAAATGAATTATGAAAATAAGGAAAC
  TTTCAAATTCTTTAAGACAGAGGCAATAAAGCAGATGGGAATTACTGCGTTGAGAACATA
  AATAAATTCCCAGTGCAGAGGACTGAACAACAGAATGTGAGTAAAAGATCTATCGCCCNN
  CCGNGGGGGCTTTTAGCTGAGGCTCTCTGTTTAAATACGTAGCCCAGTAGACTAAGTTAA
  AGATTCCAAATAGCAGCGGGAAGGCTATTCTTGACAGTCGGTCAATTTTGCTGACACTGT
  TAAAGGTTTTCTTGGGTTCTGGTGGTTTTGTTTCGGGCTTGACCTCTTTAGGTTCTATGG
  TTGCACTTTTAACAATGGTGGCTAAGCCCGGGTCGCCCCTGGCCAAATANGGGTGTANCT
  GGTTGCTGTTGGAGCGTAAGAGTTGTTTTTCTTATAAGAGGATCCTTTACTTTCTTTGCG
  CTTTCTGCAGCCCCNNAATCGCCACTCCATGCCATACCTCTCTTAATGAAATAGTTTACT
  GTGGCAAACTCAATCAGAGCTGAGAACACAAAGGCTTAGCACACGGCAATANACCATCCC
  TTAACTGGTGCCATAACCACCTTAGGGAGGGGGNTTCCTGGCACTGATGCTCAATGTTGT
  CATGGTGGAGCCCAGTTGTTCTCCAAAAACAGTCTTTGCTGGTAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000806
• Insert Size: 2290 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000806.3, NP_000797.2
• RefSeq Size: 3678 bp
• RefSeq ORF: 1371 bp
• Locus ID: 2554
• UniProt ID: P14867
• Cytogenetics: 5q34
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) uses the 5'-most alternate exon in the 5' UTR. Variants 1-7 encode the same protein.