CD64 (FCGR1A) (NM_000566) Human Untagged Clone

CAT#: SC119841

FCGR1A (untagged)-Human Fc fragment of IgG, high affinity Ia, receptor (CD64) (FCGR1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD64
• Synonyms: CD64; CD64A; FCRI; IGFR1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000566 edited
  CCAGCTTGGAGACAACATGTGGTTCTTGACAACTCTGCTCCTTTGGGTTCCAGTTGATGG
  GCAAGTGGACACCACAAAGGCAGTGATCACTTTGCAGCCTCCATGGGTCAGCGTGTTCCA
  AGAGGAAACCGTAACCTTGCACTGTGAGGTGCTCCATCTGCCTGGGAGCAGCTCTACACA
  GTGGTTTCTCAATGGCACAGCCACTCAGACCTCGACCCCCAGCTACAGAATCACCTCTGC
  CAGTGTCAATGACAGTGGTGAATACAGGTGCCAGAGAGGTCTCTCAGGGCGAAGTGACCC
  CATACAGCTGGAAATCCACAGAGGCTGGCTACTACTGCAGGTCTCCAGCAGAGTCTTCAC
  GGAAGGAGAACCTCTGGCCTTGAGGTGTCATGCGTGGAAGGATAAGCTGGTGTACAATGT
  GCTTTACTATCGAAATGGCAAAGCCTTTAAGTTTTTCCACTGGAATTCTAACCTCACCAT
  TCTGAAAACCAACATAAGTCACAATGGCACCTACCATTGCTCAGGCATGGGAAAGCATCG
  CTACACATCAGCAGGAATATCTGTCACTGTGAAAGAGCTATTTCCAGCTCCAGTGCTGAA
  TGCATCTGTGACATCCCCACTCCTGGAGGGGAATCTGGTCACCCTGAGCTGTGAAACAAA
  GTTGCTCTTGCAGAGGCCTGGTTTGCAGCTTTACTTCTCCTTCTACATGGGCAGCAAGAC
  CCTGCGAGGCAGGAACACATCCTCTGAATACCAAATACTAACTGCTAGAAGAGAAGACTC
  TGGGTTATACTGGTGCGAGGCTGCCACAGAGGATGGAAATGTCCTTAAGCGCAGCCCTGA
  GTTGGAGCTTCAAGTGCTTGGCCTCCAGTTACCAACTCCTGTCTGGTTTCATGTCCTTTT
  CTATCTGGCAGTGGGAATAATGTTTTTAGTGAACACTGTTCTCTGGGTGACAATACGTAA
  AGAACTGAAAAGAAAGAAAAAGTGGAATTTAGAAATCTCTTTGGATTCTGGTCATGAGAA
  GAAGGTAATTTCCAGCCTTCAAGAAGACAGACATTTAGAAGAAGAGCTGAAATGTCAGGA
  ACAAAAAGAAGAACAGCTGCAGGAAGGGGTGCACCGGAAGGAGCCCCAGGGGGCCACGTA
  GCAGCGGCTCAGTGGGTGGCCATCGATCTGGACCGTCCCCTGCCCACTTGCTCCCCGTGA
  GCACTGCGTACAAACATCCAAAAGTTCAACAACACCAGAACTGTGTGTCTCATGGTATAT
  AACTCTTAAAGCAAATAAATGAACTGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000566 unedited
  AGTTTAGGATATTTGTATACGACTTACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGC
  CAGCTTGGAGACACATGTGGTTCTTGACAACTCTGCTCCTTTGGGTTCCAGTTGATGGGC
  AAGTGGACACCACAAAGGCAGTGATCACTTTGCAGCCTCCATGGGTCAGCGTGTTCCAAG
  AGGAAACCGTAACCTTGCACTGTGAGGTGCTCCATCTGCCTGGGAGCAGCTCTACACAGT
  GGTTTCTCAATGGCACAGCCACTCAGACCTCGACCCCCAGCTACAGAATCACCTCTGCCA
  GTGTCAATGACAGTGGTGAATACAGGTGCCAGAGAGGTCTCTCAGGGCGAAGTGACCCCA
  TACAGCTGGAAATCCACAGAGGCTGGCTACTACTGCAGGTCTCCAGCAGAGTCTTCACGG
  AAGGAGAACCTCTGGCCTTGAGGTGTCATGCGTGGAAGGATAAGCTGGTGTACAATGTGC
  TTTACTATCGAAATGGCAAAGCCTTTAAGTTTTTCCACTGGAATTCTAACCTCACCATTC
  TGAANACCAACATAAGTCACAATGGCACCTACCATTGCTCANGCATGGGAAAGCATCGCT
  ACACATCAGCAGGAATATCTGTCACTGTGAAAGAGCTATTTCCAGCTCCAGTGCTGAATG
  CATCTGTGACATCCCCACTCCCTGGAGGGAATCTGGGTCACCTGAGCTGTGAAACANAGT
  TGCTCTTGCAGAGGCCTGGTTTGCAGCTTTACTTCTNCTTCTACATGGGCAGCAAGACCC
  TGCGAGGCANGAACACATCCTCTGAATACCAAATACTAACTGCTAGAAGAGAAGAACTCT
  GGTTATACTGNTGCGAGGCTGCCACAGAGATGGAATGTNCTNAGCGCAGCCTGAGTTGAG
  CTTCAGTGCTTGCCTCCATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000566 unedited
  CGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTGAAAGCAGTTCATTTATTTGCTTTAAAAGATTATTTACCCTGAAACA
  CACAGTTCTGGGGTTGTTGAACTTTTGGATGTTTGTACCCAATGCTCACGGGGAACAAGT
  GGGCAGGGGACGGTCCAAATCGATGGCCACCCACTGAGCCGCTGTTACGTGGCCCCCTGG
  GGCTTCTTTCGGGGCACCCCTTTCTGCAACTGGTTTTTTTTTTGGTCCTGACATTTCAAC
  TTTTTTTTTAAATGTCTGTCTTTTTGAAGGGTGGAAATTACCTTTTTTTTATGACCAAAA
  TCCAAAAAAATTTCTAAATTCCACTTTTTTTTTTTTTTCAATTCTTTACCTATTGGCACC
  CAAAAAACAGTGTTCACTAAAAACATTATTCCCACTGCCCGATAGAAAAGGACCTGAAAC
  CCGACCGGAGTTGGTAACTGGAGGCCAAACACTTGAAACTCCAACTCAGGGCTGCGCTTA
  AGGACATTTCCATCCTTTGTGGCAGCCTCCCACCAATATAACCCAAAGTCTTCTCTTTTA
  ACAGTTAATATTTGGTATTCAAAGGATGTGTTCCTGCCTCCCAGGGTCTTGCTGCCCATG
  TANAAGGAGAAGTAAAGCTGCAAACCAGGCCTCTTCAAGACAACTTTGGTTACAGCTTCA
  GGTGACCAAATTCCCCTCCAGAGTGGGGATGCACAGATGCCTTCAGCACTGGACCTGGAA
  TAACTCTTTCCAAGGACACGAATTCCTGCTGATGGTAACGAAGTTTTCCTTGCCGAGCAA
  GGGAGCGCCACTGGGACTTAGTGTGGTTTCAAAATGGGAGGTTAAAATTCAGGGGAAAAC
  TTAAAGCTTGGCTTTTCAATGTAACCATTGTACACACTTTTCCTTCCGCCTGACCCTAGA
  GCCCAGGTTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000566
• Insert Size: 1300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000566.2, NP_000557.1
• RefSeq Size: 2230 bp
• RefSeq ORF: 1125 bp
• Locus ID: 2209
• UniProt ID: P12314
• Cytogenetics: 1q21.2
• Domains: ig, IGc2, IG
• Protein Families: Transmembrane
• Protein Pathways: Fc gamma R-mediated phagocytosis, Hematopoietic cell lineage, Systemic lupus erythematosus
• Gene Summary: This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]