Home Destination Vector pCMV6-AN-mBFP
pCMV6-AN-mBFP, mammalian vector with N-terminal mBFP , 10ug
- ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal mBFP (monomeric) tag.
- mBFP (scientific name mTagBFP) is a monomeric blue fluorescent protein. mBFP possesses bright blue fluorescence with excitation/emission maxima at 402 and 457 nm, characterized by high photostability and extremely high pH-stability.
Compared to EBFP2, mBFP is more than 1.8 times brighter, much more pH-stable and has twice shorter maturation half-time at 37°C. Narrow fluorescence emission peak of mBFP provides for accurate and easy spectral separation with cyan and green fluorescent proteins and makes it a preferable tag for multicolor labeling.
Good overlap between the emission spectrum of mBFP and the absorbance spectra of mGFP allows using these two proteins as a FRET pair.
- If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal mBFP-tagging vector, PS100043.
Schematic of the multiple cloning sites:
OriGene's fluorescent protein genes are all licensed from Evrogen. Plasmid vectors harboring a fluorescent protein gene contains a proprietary nucleic acid coding for a proprietary fluorescent proteins(s) intended to be used for not-for-profit internal research purposes only, where "research purposes" means non-conmmercial uses or activities which (or the results of which) do not generate revenue. Any use of the proprietary nucleic acid or protein other than for research use is strictly prohibited. USE IN ANY OTHER APPLICATION REQUIRES A LICENSE FROM EVROGEN. To obtain such a license, please contact Evrogen at firstname.lastname@example.org