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Home Destination Vector pCMV6-AN-mRFP
PrecisionShuttle mammalian vector with N-terminal mRFP
Features:
- ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal mRFP (monomeric) tag.
- mRFP is a monomeric red (orange) fluorescent protein generated from the wild-type RFP from sea anemone Entacmaea quadricolor. It possesses bright fluorescence with excitation/emission maxima at 555 and 584 nm, respectively. mRFP is about three times brighter than mCherry protein, which makes it the brightest monomeric red fluorescent protein available so far.
mRFP is mainly intended for protein labeling and FRET applications. It can also be used for cell and organelle labeling and for tracking the promoter activity. - If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal mRFP-tagging vector, PS100041.
Schematic of the multiple cloning sites:
OriGene's fluorescent protein genes are all licensed from Evrogen. Plasmid vectors harboring a fluorescent protein gene contains a proprietary nucleic acid coding for a proprietary fluorescent proteins(s) intended to be used for not-for-profit internal research purposes only, where "research purposes" means non-conmmercial uses or activities which (or the results of which) do not generate revenue. Any use of the proprietary nucleic acid or protein other than for research use is strictly prohibited. USE IN ANY OTHER APPLICATION REQUIRES A LICENSE FROM EVROGEN. To obtain such a license, please contact Evrogen at license@evrogen.com.
All OriGene’s TrueORFs inserts can be subcloned into this vector via custom cloning service. To use this service:
- Find the gene of interest using any of the Gene Name, Gene Symbol, Accession Number or Keyword.
- At the product page displaying the correct gene of interest, choose the appropriate destination vector and select the “Custom Cloning” .
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