OriGene - pCMV6-AN-mGFP Destination Vector

Features:

ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal mGFP (monomeric) tag.
mGFP (scientific name mTagGFP) is the improved variant of tagGFP, the mutant of the Aequorea macrodactyla GFP-like protein. mGFP possesses bright green fluorescence with excitation/emission maxima at 483 and 506 nm, respectively.
Compared to EGFP, mGFP provides about the same brightness of fluorescence but is significantly more pH stable. mGFP is specially optimized for expression at 37°C.
Because of monomeric nature, mGFP is mainly intended for protein localization studies and expression in long-term cell cultures. In FRET applications, mGFP can be used as a donor for red fluorescent protein mRFP or as an acceptor for blue fluorescent protein mBFP.
If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal mGFP-tagging vector, PS100040.

PrecisionShuttle mammalian vector with N-terminal mGFP Vector Image

Schematic of the multiple cloning sites:

PrecisionShuttle mammalian vector with N-terminal mGFP MCS Image

Download full vector sequence

OriGene's fluorescent protein genes are all licensed from Evrogen. Plasmid vectors harboring a fluorescent protein gene contains a proprietary nucleic acid coding for a proprietary fluorescent proteins(s) intended to be used for not-for-profit internal research purposes only, where "research purposes" means non-conmmercial uses or activities which (or the results of which) do not generate revenue. Any use of the proprietary nucleic acid or protein other than for research use is strictly prohibited. USE IN ANY OTHER APPLICATION REQUIRES A LICENSE FROM EVROGEN. To obtain such a license, please contact Evrogen at license@evrogen.com.

All OriGene’s TrueORFs inserts can be subcloned into this vector via custom cloning service. To use this service:

Find the gene of interest using any of the Gene Name, Gene Symbol, Accession Number or Keyword.

Search:
At the product page displaying the correct gene of interest, choose the appropriate destination vector and select the “Custom Cloning” .

PrecisionShuttle mammalian vector with N-terminal mGFP