OriGene - pCMV6-AC-FP635 Destination Vector

Features:

ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal TurboFP635 tag.
TurboFP635 (scientific name Katushka) is a far-red mutant of the red fluorescent protein from sea anemone Entacmaea quadricolor. Possessing excitation/emission maxima at 588/635 nm, TurboFP635 is 7 to 10-fold brighter compared to the spectrally close or mPlum. TurboFP635 is characterized by fast maturation and a high pH-stability and photostability. The unique characteristics of TurboFP635 make it the protein of choice for visualization within living tissues and dual-color high-throughput assays.
TurboFP635 is mainly intended for applications where fast appearance of far-red fluorescence is crucial. It is specially recommended for whole body imaging, cell and organelle labeling, and for tracking the promoter activity in auto-fluorescent tissues.
If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal FP635-tagging vector, PS100046.

PrecisionShuttle mammalian vector with C-terminal FP635 tag Vector Image

Schematic of the multiple cloning sites:

PrecisionShuttle mammalian vector with C-terminal FP635 tag MCS Image

Download full vector sequence

OriGene's fluorescent protein genes are all licensed from Evrogen. Plasmid vectors harboring a fluorescent protein gene contains a proprietary nucleic acid coding for a proprietary fluorescent proteins(s) intended to be used for not-for-profit internal research purposes only, where "research purposes" means non-conmmercial uses or activities which (or the results of which) do not generate revenue. Any use of the proprietary nucleic acid or protein other than for research use is strictly prohibited. USE IN ANY OTHER APPLICATION REQUIRES A LICENSE FROM EVROGEN. To obtain such a license, please contact Evrogen at license@evrogen.com.

All OriGene’s TrueORFs inserts can be subcloned into this vector via custom cloning service. To use this service:

Find the gene of interest using any of the Gene Name, Gene Symbol, Accession Number or Keyword.

Search:
At the product page displaying the correct gene of interest, choose the appropriate destination vector and select the “Custom Cloning” .

PrecisionShuttle mammalian vector with C-terminal FP635 tag